Swine Leukocyte Antigen Diversity in Canadian Specific Pathogen-Free Yorkshire and Landrace Pigs.
Swine Leukocyte Antigen Diversity in Canadian Specific Pathogen-Free Yorkshire and Landrace Pigs.
复制标题
加拿大特定无病原体约克夏猪和长白猪的猪白细胞抗原多样性
DOI：
10.3389/fimmu.2017.00282
复制
发表时间：
2017
影响因子：
7.3
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Gao C;Quan J;Jiang X;Li C;Lu X;Chen H
猪主要组织相容性复合体（MHC）是一种高度多态性的抗原，被称为猪白细胞抗原（SLA），与猪对传染病、疫苗和移植的不同免疫应答水平有关。具有已知SLA单倍型的猪种是生物医学研究的重要遗传资源。加拿大约克郡猪和长白猪代表目前在中国农业科学院哈尔滨兽医研究所隔离环境中饲养的无特定病原体（SPF）种猪。在这项研究中，我们确定了61个等位基因在5个多态性SLA位点（SLA-1，SLA-2，SLA-3，DRB 1，和DQB 1）代表17个I类单倍型和11个II类单倍型，使用逆转录-聚合酶链反应（RT-PCR）基于序列的分型和PCR序列特异性引物方法在367加拿大SPF约克郡和长白猪。等位基因的正式名称由国际动物遗传学会SLA命名委员会指定，并在更新的免疫多态性数据库-MHC SLA序列数据库中发布[发布2.0.0.3（2016-11-03）]。提交的资料证实了一些未分配的等位基因，并标准化了GenBank数据库中许多以前未确认的等位基因的命名。三个I类单倍型，Hp-37.0，63.0和73.0，似乎是新的，以前没有在其他猪群体中报道。观察到I类区域内的一个交叉和I类和II类区域之间的两个交叉，导致三个新的重组单倍型。重复的SLA-1基因座的存在被证实在三个I类单倍型Hp-28.0，Hp-35.0，和Hp-63.0。此外，我们还分析了19个在本研究中鉴定的常见SLA I类分子的功能差异，并使用MHC簇方法证实了四个超型的存在。这些结果将有助于研究猪的适应性免疫反应和免疫表型差异，筛选潜在的T细胞表位，并进一步开发更有效的疫苗。
The highly polymorphic swine major histocompatibility complex (MHC), termed swine leukocyte antigen (SLA), is associated with different levels of immunologic responses to infectious diseases, vaccines, and transplantation. Pig breeds with known SLA haplotypes are important genetic resources for biomedical research. Canadian Yorkshire and Landrace pigs represent the current specific pathogen-free (SPF) breeding stock maintained in the isolation environment at the Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences. In this study, we identified 61 alleles at five polymorphic SLA loci (SLA-1, SLA-2, SLA-3, DRB1, and DQB1) representing 17 class I haplotypes and 11 class II haplotypes using reverse transcription-polymerase chain reaction (RT-PCR) sequence-based typing and PCR-sequence specific primers methods in 367 Canadian SPF Yorkshire and Landrace pigs. The official designation of the alleles has been assigned by the SLA Nomenclature Committee of the International Society for Animal Genetics and released in updated Immuno Polymorphism Database-MHC SLA sequence database [Release 2.0.0.3 (2016-11-03)]. The submissions confirmed some unassigned alleles and standardized nomenclatures of many previously unconfirmed alleles in the GenBank database. Three class I haplotypes, Hp-37.0, 63.0, and 73.0, appeared to be novel and have not previously been reported in other pig populations. One crossover within the class I region and two between class I and class II regions were observed, resulting in three new recombinant haplotypes. The presence of the duplicated SLA-1 locus was confirmed in three class I haplotypes Hp-28.0, Hp-35.0, and Hp-63.0. Furthermore, we also analyzed the functional diversities of 19 identified frequent SLA class I molecules in this study and confirmed the existence of four supertypes using the MHCcluster method. These results will be useful for studying the adaptive immune response and immunological phenotypic differences in pigs, screening potential T-cell epitopes, and further developing the more effective vaccines.