Variability of the caprine whey protein genes and their association with milk yield, composition and renneting properties in the Sarda breed. 1. The LALBA gene
Variability of the caprine whey protein genes and their association with milk yield, composition and renneting properties in the Sarda breed. 1. The LALBA gene
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DOI：
10.1017/s0022029915000461
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发表时间：
2015-11-01
影响因子：
2.1
通讯作者：
Vacca, Giuseppe Massimo
中科院分区：
文献类型：
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作者：
Dettori, Maria Luisa;Pazzola, Michele;Vacca, Giuseppe Massimo
对山羊LALBA基因5'端侧翼区和3'端非翻译区进行了SSCP分析和序列测定。共检测到9个SNP：3个在启动子区，2个是外显子1的同义编码SNP，4个SNP在外显子4的3 'UTR内。核苷酸变化位于启动子区（c.- 358T>C，c.- 163G>A，c.-用SSCP方法对263只萨尔达山羊进行了121 T>G）基因分型，以研究其对产奶量、乳成分和凝乳性能的影响。我们观察了这三个SNP对产奶量和乳糖含量的影响。基因型TT和CT在c. 358 T>C（P < 0.001），c.-358 T> C基因型AG和GG 163 G>A（P < 0.01）的乳糖含量较高，而c. 358 CC和C.- 163 AA组产奶量极显著低于对照组（P < 0.01）。SNPs c.- 358 T>C和C.- 121 T>G是转录因子结合位点的一部分，可能参与调节LALBA基因的表达。LALBA基因型影响凝乳特性（P < 0.001），杂合子c.- 358 CT和C.- 163 GA的凝乳酶凝固时间和凝乳硬化时间延迟，凝乳硬度最低。本研究增加了SNPs的面板，并增加了山羊LALBA基因多态性的影响的新信息。
The 5' flanking region and 3' UTR of the caprine LALBA gene were analysed by SSCP and sequencing. A total of nine SNPs were detected: three in the promoter region, two were synonymous coding SNPs at exon-1, and four SNPs were in exon-4, within the 3'UTR. The nucleotide changes located in the promoter region (c.-358T>C, c.-163G>A, c.-121T>G) were genotyped by SSCP in 263 Sarda goats to evaluate their possible effect on milk yield, composition and renneting properties. We observed an effect of the three SNPs on milk yield and lactose content. Genotypes TT and CT at c.-358T>C (P < 0.001) and genotypes AG and GG at c.-163G>A (P < 0.01) were characterised by higher lactose contents, while c.-358CC and c.-163AA showed the lower milk yield (P < 0.01). SNPs c.-358T>C and c.-121T>G were part of transcription factors binding sites, potentially involved in modulating the LALBA gene expression. The LALBA genotype affected renneting properties (P < 0.001), as heterozygotes c.-358CT and c.-163GA were characterised by delayed rennet coagulation time and curd firming time and the lowest value of curd firmness. The present investigation increases the panel of SNPs and adds new information about the effects of the caprine LALBA gene polymorphism.