Proteomics investigation of endogenous S-nitrosylation in Arabidopsis
Proteomics investigation of endogenous S-nitrosylation in Arabidopsis
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DOI：
10.1016/j.bbrc.2011.11.036
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发表时间：
2011-12-16
影响因子：
3.1
通讯作者：
Peltier, Jean-Benoit
中科院分区：
文献类型：
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作者：
Fares, Abasse;Rossignol, Michel;Peltier, Jean-Benoit
s -亚硝基化作为一种重要的蛋白质修饰在许多过程中出现。然而，大多数数据是在添加NO供体后在蛋白质水平上获得的，特别是在关于这些蛋白质中亚硝基化半胱氨酸的信息很少的植物中。提出了一种将经典生物素转换方法与同位素编码亲和标签（ICAT）相结合的工作流程。在不添加NO供体的情况下，在拟南芥细胞中共鉴定出53种内源性亚硝基半胱氨酸，这些蛋白质属于所有细胞区域，包括膜，并覆盖了大量功能。植物中亚硝基硫醇的第一个曲目也使初步的结构描述成为可能。三个极性基序，不位于半胱氨酸附近，占数据集的一半，被检测到，并提出了补充亚硝基化预测算法，与迄今为止的植物数据训练不足。短暂盐胁迫下的亚硝基化变化分析表明，NaCl改变了一小部分内源性亚硝基化蛋白的亚硝基化水平，并没有影响到这些蛋白中的所有亚硝基硫醇。讨论了一些NO靶点在盐胁迫响应中的可能作用。(C) 2011爱思唯尔公司版权所有。
S-Nitrosylation emerges as an important protein modification in many processes. However, most data were obtained at the protein level after addition of a NO donor, particularly in plants where information about the cysteines nitrosylated in these proteins is scarce. An adapted work-flow, combining the classical biotin switch method and labeling with isotope-coded affinity tags (ICAT), is proposed. Without addition of NO donor, a total of 53 endogenous nitrosocysteines was identified in Arabidopsis cells, in proteins belonging to all cell territories, including membranes, and covering a large panel of functions. This first repertoire of nitrosothiols in plants enabled also preliminary structural description. Three apolar motifs, not located in close vicinity of cysteines and accounting for half the dataset, were detected and are proposed to complement nitrosylation prediction algorithms, poorly trained with plant data to date. Analysis of changes induced by a brief salt stress showed that NaCl modified the nitrosylation level of a small proportion of endogenously nitrosylated proteins and did not concern all nitrosothiols in these proteins. The possible role of some NO targets in the response to salt stress was discussed. (C) 2011 Elsevier Inc. All rights reserved.