A novel electrochemical aptasensor for ultrasensitive detection of herbicide prometryn based on its highly specific aptamer and Ag@Au nanoflowers.
A novel electrochemical aptasensor for ultrasensitive detection of herbicide prometryn based on its highly specific aptamer and Ag@Au nanoflowers.
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DOI：
10.1016/j.talanta.2023.124838
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发表时间：
2023-06
期刊：
影响因子：
6.1
通讯作者：
Zhenzhong Zhang;Yunxia Luan;S. Ru;Hayan Teng;Yuejiao Li;Minhao Liu;Jun Wang
中科院分区：
文献类型：
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作者：
Zhenzhong Zhang;Yunxia Luan;S. Ru;Hayan Teng;Yuejiao Li;Minhao Liu;Jun Wang
除草剂promedyn已成为水生环境中常见的污染物，对生态系统造成了不利影响。本研究基于其高亲和性和特异性的适体和用于信号放大的Ag@Au纳米花（Ag@AuNFs），开发了一种超灵敏的prometryn电化学适体传感器。首先，本研究改进了Capture-SELEX筛选适配体的策略，获得了具有高亲和力（Kd: 23 nM）的适配体P60-1，该适配体可以将promeyn与其结构类似物区分开来。此外，在P60-1适体中发现典型的茎环结构是prometryn的结合袋。随后，以多壁碳纳米管和还原氧化石墨烯为电极衬底，Ag@Au NFs为信号放大元件，适配体P60-1为识别元件，建立了一种用于prometryn的电化学适配体传感器。该传感器的检测范围为0.16 ~ 500 ng/mL，检出限为60 pg/mL，远低于现有的检测方法。该传感器稳定性高，重复性好，能特异性地检测prometryn。此外，通过测量环境和生物成分中的prometryn，验证了该传感器的实用性。因此，本研究提供了一种鲁棒性和超灵敏的适体传感器，用于准确检测prome畏污染。
Herbicide prometryn has become a common pollutant in aquatic environments and caused adverse impacts on ecosystems. This study developed an ultrasensitive electrochemical aptasensor for prometryn based on its highly affinitive and specific aptamer and Ag@Au nanoflowers (Ag@AuNFs) for signal amplification. Firstly, this study improved the Capture-SELEX strategy to screen aptamers and obtained aptamer P60-1, which had a high affinity (Kd: 23 nM) and could distinguish prometryn from its structural analogues. Moreover, the typical stem-loop structure in aptamer P60-1 was found to be the binding pocket for prometryn. Subsequently, an electrochemical aptasensor for prometryn was established using multiwalled carbon nanotubes and reduced graphene oxide as electrode substrate, Ag@Au NFs as signal amplification element, and aptamer P60-1 as recognition element. The aptasensor had a detection range of 0.16–500 ng/mL and a detection limit of 60 pg/mL, which was much lower than those of existing detection methods. The aptasensor had high stability and good repeatability, and could specifically detecting prometryn. Furthermore, the utility of the aptasensor was validated by measuring prometryn in environmental and biological components. Therefore, this study provides a robust and ultrasensitive aptasensor for accurate detection for prometryn pollution.