The spatio-temporal dynamics of ligament healing.
The spatio-temporal dynamics of ligament healing.
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DOI：
10.1111/j.1524-475x.2009.00465.x
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
Vanderby R
中科院分区：
文献类型：
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作者：
Chamberlain CS;Crowley E;Vanderby R
韧带损伤通常无法有效治疗以恢复其原始状态。许多研究检查了受伤后伤口的愈合情况，报告了伤口内的临时基质和疤痕形成。然而，很少有研究以时空方式报道韧带愈合过程中的炎症、增殖和重塑过程。我们的目标是在大鼠内侧副韧带 (MCL) 愈合模型中更全面地阐明这一过程。在这项研究中，通过手术横断内侧副韧带并使其愈合。在损伤后1、3、5、7、9、11、14和28天收集韧带并用微血管造影或免疫组织化学检查。我们证明中性粒细胞和有丝分裂细胞在损伤后 1 至 5 天之间达到峰值。大多数因素在损伤后 5 至 9 天之间达到顶峰，包括循环巨噬细胞、常驻巨噬细胞、T 淋巴细胞、造血细胞、血管内皮生长因子和血管。从第 9 天到研究结束（第 28 天），凋亡细胞占主导地位。最初，大多数测定的标记物定位于损伤部位的表皮韧带和肉芽组织。随后，具有肉芽组织和细胞的愈合区域继续扩展到未受伤的组织中。根据这些结果，我们扩展了当前对韧带愈合的描述，并提供了愈合过程的更完整的表示。
Ligament injury commonly occurs with no effective treatment to restore its original state. Numerous studies have examined wound healing after injury, reporting a provisional matrix and scar formation within the wound. Few studies however report the inflammatory, proliferative, and remodeling process during ligament healing in a spatio-temporal manner. Our goal was then to more completely elucidate this process in a rat medial collateral ligament (MCL) healing model. In this study, medial collateral ligaments were surgically transected and allowed to heal. At 1, 3, 5, 7, 9, 11, 14, and 28 days postinjury ligaments were collected and examined with microangiography or immunohistochemistry. We demonstrate that neutrophils and mitotic cells peak between 1 and 5 days postinjury. The majority of factors crest between 5 and 9 days postinjury, including circulating macrophages, resident macrophages, T lymphocytes, hematopoietic cells, vascular endothelial growth factor, and blood vessels. The apoptotic cells predominate from day 9 to the end of the study (day 28). Initially, most assayed markers localize to the epiligament and to granulation tissue at the site of damage. Later, the healing region with its granulation tissue and cells continues to expand into the uninjured tissue. From these results, we have expanded current descriptions of ligament healing and offer a more complete representation of the healing process.