Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2
Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2
复制标题
DOI：
10.1163/15685411-00002875
复制
发表时间：
2015
期刊：
影响因子：
1.2
通讯作者：
Yu Mei;P. Thorpe;Athanas Guzha;A. Haegeman;V. Blok;K. MacKenzie;G. Gheysen;John T Jones;S. Mantelin
中科院分区：
文献类型：
--
作者：
Yu Mei;P. Thorpe;Athanas Guzha;A. Haegeman;V. Blok;K. MacKenzie;G. Gheysen;John T Jones;S. Mantelin
对马铃薯孢囊线虫Globodera pallida的基因组序列分析表明，该物种中存在一个含有spry结构域的重要基因家族(约300个序列)。与包括其他植物寄生线虫在内的其他生物相比，这是该基因家族的一个巨大扩张。苍白球藻和罗氏球藻的一些spry结构域蛋白具有分泌信号肽，并作为效应物部署。其中一种SPRYSEC蛋白已被证明能抑制宿主防御反应。我们描述了这一基因家族在苍白球虫中的进一步分析。我们发现，该物种中只有一小部分(10%)的spry结构域蛋白具有预测的分泌信号肽，并且信号肽的存在与寄生早期表达的相应基因密切相关。这些数据表明，虽然基因家族得到了极大的扩展，但在苍白球虫中只有一小部分spry结构域蛋白是SPRYSEC候选效应器。我们发现了几个新的SPRYSECs在背腺细胞中表达，并证明了部分但不是全部SPRYSECs可以抑制由抗性基因Gpa2及其同源无毒因子RBP-1在本山烟草中共表达而引起的过敏反应。
Analysis of the genome sequence of the potato cyst nematode, Globodera pallida, has shown that a substantial gene family (approximately 300 sequences) of proteins containing a SPRY domain is present in this species. This is a huge expansion of the gene family as compared to other organisms, including other plant-parasitic nematodes. Some SPRY domain proteins from G. pallida and G. rostochiensis have signal peptides for secretion and are deployed as effectors. One of these SPRYSEC proteins has been shown to suppress host defence responses. We describe further analysis of this gene family in G. pallida. We show that only a minority (10%) of the SPRY domain proteins in this species have a predicted signal peptide for secretion and that the presence of a signal peptide is strongly correlated with the corresponding gene being expressed at the early stages of parasitism. The data suggest that while the gene family is greatly expanded, only a minority of SPRY domain proteins in G. pallida are SPRYSEC candidate effectors. We show that several new SPRYSECs from G. pallida are expressed in the dorsal gland cell and demonstrate that some, but not all, of the SPRYSECs can suppress the hypersensitive response induced by co-expression of the resistance gene Gpa2 and its cognate avirulence factor RBP-1 in Nicotiana benthamiana.