The functional response of U937 macrophage-like cells is modulated by extracellular matrix proteins and mechanical strain
The functional response of U937 macrophage-like cells is modulated by extracellular matrix proteins and mechanical strain
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DOI：
10.1139/o06-093
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发表时间：
2006-10-01
影响因子：
2.9
通讯作者：
Labow, Rosalind S.
中科院分区：
文献类型：
--
作者：
Matheson, Loren A.;Maksym, Geoffrey N.;Labow, Rosalind S.
细胞外基质蛋白（ECMs）在机械应变转移到单核细胞源性巨噬细胞（MDMs）中发挥重要作用，影响异物反应的形态变化。本研究考察了U937巨噬细胞样细胞在涂有I型胶原或RGD肽重复序列（ProNectin((R))）的硅氧烷膜上培养时，在2种动态菌株类型（非均匀双轴菌株或均匀单轴菌株）下的功能反应差异。双轴菌株引起细胞内酯酶和酸性磷酸酶（AP）活性的增加，以及单核细胞特异性酯酶（MSE）蛋白水平的增加，在未包被的表面（如前所示）或胶原蛋白上播种，但没有ProNectin（(R)）。释放AP活性增加，但未释放酯酶活性。双轴应变使所有表面的IL-6升高，但IL-8不升高。当细胞受到单轴应变时，细胞内酯酶仅在被涂表面增加，而细胞内AP活性不受影响。所有表面的酯酶和AP释放活性均增加。单轴应变增加了IL-6在所有表面上的释放，而IL-8仅在涂油表面释放。本研究首次证明了ECM蛋白可以特异性调节细胞对不同类型菌株的反应。在体外细胞系统中使用这种方法可能有助于揭示MDMs在异物反应中的复杂功能。
Extracellular matrix proteins (ECMs) play a significant role in the transfer of mechanical strain to monocyte-derived macrophages (MDMs) affecting morphological changes in a foreign body reaction. This study investigated how the functional responses of U937 macrophage-like cells differed when subjected to 2 dynamic strain types (nonuniform biaxial or uniform uniaxial strain) while cultured on siloxane membranes coated with either collagen type I or RGD peptide repeats (ProNectin((R))). Biaxial strain caused an increase in intracellular esterase and acid phosphatase (AP) activities, as well as monocyte-specific esterase (MSE) protein levels in cells that were seeded on either uncoated surfaces (shown previously) or collagen, but not ProNectin((R)). Released AP activity, but not released esterase activity, was increased on all surfaces. Biaxial strain increased IL-6 but not IL-8 on all surfaces. When cells were subjected to uniaxial strain, intracellular esterase increased on coated surfaces only, whereas intracellular AP activity was unaffected. Both esterase and AP released activities increased on all surfaces. Uniaxial strain increased the release of IL-6 on all surfaces, but IL-8 oil coated surfaces only. This study demonstrated for the first time that ECM proteins Could specifically modulate cellular responses to different types of strain. Using this approach with an in vitro cell system may help to unravel the complex function of MDMs in the foreign-body reaction.