Transcriptomic Analysis of Laribacter hongkongensis Reveals Adaptive Response Coupled with Temperature.
Transcriptomic Analysis of Laribacter hongkongensis Reveals Adaptive Response Coupled with Temperature.
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DOI：
10.1371/journal.pone.0169998
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Lau TC
中科院分区：
文献类型：
--
作者：
Kong HK;Law HW;Liu X;Law CO;Pan Q;Gao L;Xiong L;Lau SK;Woo PC;Lau TC
细菌对不同宿主的适应需要转录组的改变以响应环境条件。香港海鸥形杆菌是一种革兰氏阴性、兼性厌氧、尿素酶阳性杆菌，可引起肝硬化及社区获得性胃肠炎患者感染。在一般食用的淡水鱼和饮用水库的肠道中也发现了它。自L. hongkongensis既可以作为鱼类病原体生存，也可以作为人类病原体生存，它们在两种不同生境中的生存机制应该是受温度调节的，并且是高度复杂的。因此，我们对L. hongkongensis在鱼类和人类体温下的变化，以阐明与温度耦合的多种适应机制。我们确定了许多新的温度诱导的途径参与主机的发病机制，除了代谢平衡的转变和应激相关蛋白的过表达。此外，这些途径形成了一个网络，可以在特定温度下激活，并改变细菌的生理机能以适应环境。总之，L. hongkongensis为细菌在不同生境的生存提供了多种策略，这种改变使细菌为挑战宿主免疫力做好了准备。
Bacterial adaptation to different hosts requires transcriptomic alteration in response to the environmental conditions. Laribacter hongkongensis is a gram-negative, facultative anaerobic, urease-positive bacillus caused infections in liver cirrhosis patients and community-acquired gastroenteritis. It was also found in intestine from commonly consumed freshwater fishes and drinking water reservoirs. Since L. hongkongensis could survive as either fish or human pathogens, their survival mechanisms in two different habitats should be temperature-regulated and highly complex. Therefore, we performed transcriptomic analysis of L. hongkongensis at body temperatures of fish and human in order to elucidate the versatile adaptation mechanisms coupled with the temperatures. We identified numerous novel temperature-induced pathways involved in host pathogenesis, in addition to the shift of metabolic equilibriums and overexpression of stress-related proteins. Moreover, these pathways form a network that can be activated at a particular temperature, and change the physiology of the bacteria to adapt to the environments. In summary, the dynamic of transcriptomes in L. hongkongensis provides versatile strategies for the bacterial survival at different habitats and this alteration prepares the bacterium for the challenge of host immunity.