Effects of phosphorylation on the activity of glycogen phosphorylase in mutton during incubation at 4 °C in vitro
Effects of phosphorylation on the activity of glycogen phosphorylase in mutton during incubation at 4 °C in vitro
复制标题
4℃体外培养过程中磷酸化对羊肉糖原磷酸化酶活性的影响
DOI：
10.1016/j.foodchem.2020.126162
复制
发表时间：
2020-05-01
期刊：
影响因子：
8.8
通讯作者：
Ren，Chi
中科院分区：
文献类型：
--
作者：
Bai，Yuqiang;Li，Xin;Ren，Chi
本研究的目的是评估在4 °C孵育期间，糖原磷酸化酶的磷酸化水平对其在羊肉肌浆蛋白样品中的活性的影响。制备了来自最长肌胸肌的肌浆蛋白样品，并将其分成三个处理组以获得具有不同磷酸化水平的糖原磷酸化酶，所述处理组（1）用蛋白激酶A处理，（2）用碱性磷酸酶处理，和（3）不处理（对照）。评估糖原磷酸化酶磷酸化水平和活性以及相关内源性物质水平。结果表明，在4 °C孵育过程中，羊肉糖原磷酸化酶的磷酸化促进了其活性。糖原磷酸化酶活性还受其他因素（糖原、葡萄糖、葡萄糖6-磷酸、ATP等）的影响。体外磷酸化和内源性物质的联合作用对糖原磷酸化酶活性的影响在不同的孵育时间不同。
The aim of this study was to assess the effects of the phosphorylation levels of glycogen phosphorylase on its activity in mutton sarcoplasmic protein samples during incubation at 4 °C. Samples of sarcoplasmic proteins from muttonlongissimus thoracismuscles were prepared and separated into three treatment groups to obtain glycogen phosphorylase with different phosphorylation levels, which were (1) treated with protein kinase A, (2) treated with alkaline phosphatase, and (3) left untreated (control). Glycogen phosphorylase phosphorylation levels and activity as well as the levels of related endogenous substances were assessed. The results showed that phosphorylation of glycogen phosphorylase in mutton promoted its activity during incubation at 4 °C. The activity of glycogen phosphorylase was also influenced by other factors (glycogen, glucose, glucose 6-phosphate, ATP, etc.)in vitro. The combined effects of phosphorylation and endogenous substances on glycogen phosphorylase activity varied at different incubation times.