Large-scale gene discovery in the oomycete Phytophthora infestans reveals likely components of phytopathogenicity shared with true fungi
Large-scale gene discovery in the oomycete Phytophthora infestans reveals likely components of phytopathogenicity shared with true fungi
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DOI：
10.1094/mpmi-18-0229
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发表时间：
2005-03-01
影响因子：
3.5
通讯作者：
Judelson, HS
中科院分区：
文献类型：
--
作者：
Randall, TA;Dwyer, RA;Judelson, HS
为了全面了解马铃薯晚疫病菌的基因内容，对马铃薯晚疫病菌进行了大规模cDNA和基因组测序。从20个cDNA文库中获得了一组来自马铃薯晚疫病菌的75，757个高质量表达序列标签（EST），这些cDNA文库代表了广泛的生长条件、胁迫反应和发育阶段。这些包括从马铃薯和番茄的相互作用，其中963病原体EST确定的R infestans库。为了补充EST，获得了马铃薯晚疫病菌基因组的单倍覆盖率，并鉴定了编码潜力区域。从EST和基因组数据中获得了18，256个序列的单基因组，并表征了潜在功能、阶段特异性表达模式和密码子偏好。EST聚类分析显示菌丝和孢子相关阶段的表达基因含量之间存在较大差异，并且某些生长条件之间存在亲和力。真菌致病性基因的数据库的比较显示保守的致病性元素，如III类果胶裂解酶，尽管卵菌和真菌之间的进化距离相当大。还鉴定了37个编码鞭毛组分的基因。检测到几个预期不会发生在卵菌中的基因，包括几丁质酶、磷酸激酶和细菌型FtsZ细胞分裂蛋白。所描述的序列数据可在可搜索的公共数据库中获得。
To overview the gene content of the important pathogen Phytophthora infestans, large-scale cDNA and genomic sequencing was performed. A set of 75,757 high-quality expressed sequence tags (ESTs) from P infestans was obtained from 20 cDNA libraries representing a broad range of growth conditions, stress responses, and developmental stages. These included libraries from R infestans-potato and -tomato interactions, from which 963 pathogen ESTs were identified. To complement the ESTs, onefold coverage of the P infestans genome was obtained and regions of coding potential identified. A unigene set of 18,256 sequences was derived from the EST and genomic data and characterized for potential functions, stage-specific patterns of expression, and codon bias. Cluster analysis of ESTs revealed major differences between the expressed gene content of mycelial and spore-related stages, and affinities between some growth conditions. Comparisons with databases of fungal pathogenicity genes revealed conserved elements of pathogenicity, such as class III pectate lyases, despite the considerable evolutionary distance between oomycetes and fungi. Thirty-seven genes encoding components of flagella also were identified. Several genes not anticipated to occur in oomycetes were detected, including chitin synthases, phosphagen kinases, and a bacterial-type FtsZ cell-division protein. The sequence data described are available in a searchable public database.