Preservation of perisomatic inhibitory input of granule cells in the epileptic human dentate gyrus
Preservation of perisomatic inhibitory input of granule cells in the epileptic human dentate gyrus
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DOI：
10.1016/s0306-4522(01)00446-8
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Freund, TF
中科院分区：
文献类型：
--
作者：
Wittner, L;Maglóczky, Z;Freund, TF
已知颞叶癫痫与在海马形成中可能产生或扩增的多动症有关。大多数颗粒细胞，齿状回的主要细胞被发现对癫痫的损伤具有抵抗力。如果癫痫发作的抑制作用受损，则可以作为发电机。因此，在人类对照和癫痫性齿状回检查中检查了含颗粒细胞somata和轴突初始段的最强大抑制性输入的中间神经元的子集。在癫痫样品中发现了含白蛋白白细胞蛋白细胞的数量的大幅度减少，尤其是在Hilar区域中，尽管在颗粒细胞层的某些斑块中，白细胞蛋白白蛋白阳性末端是形成轴向轴垂直簇特征的轴突细胞特征比对照组大的多数。 。对对照组中的白细胞蛋白阳性轴突末端的突触后目标元素的分析表明，它们与somata，树突，轴突初始片段和棘突形成对称突触，但在癫痫组织中，轴突初始段突触的比率增加（对照组织：控制：： 15.9％，癫痫病：31.3％）。此外。在癫痫样品中，颗粒细胞轴突初始片段的突触覆盖量增加了三倍以上（对照：0.52，癫痫：2.10 MUM突触长度/100 MUM轴突初始段膜），而体细胞对称突触的量没有显着变化。尽管白蛋白阳性阳性中间神经元的数量减少，但牙齿颗粒细胞的par骨抑制输入仍保留在颞叶癫痫中。篮子和轴心轴突细胞末端（无论是白蛋白蛋白的阳性还是阴性），此外，靶向轴突初始段的轴突侧支靶向癫痫齿状回的轴突初始片段发芽。我们建议诊断性抑制性抑制性中间神经元在癫痫中存活，但它们的体积隔离和部分隔离型隔离阶层和部分隔离型隔离阶层。轴突失去白蛋白或免疫反应性白蛋白。轴突初始片段的过度接触可能是抑制网络的补偿性变化，但与此同时，可能导致颗粒细胞发射的更有效的同步，这可能有助于癫痫发作的产生或扩增。 （c）2001 IBRO。由Elsevier Science Ltd.出版。保留所有权利。
Temporal lobe epilepsy is known to be associated with hyperactivity that is likely to be generated or amplified in the hippocampal formation. The majority of granule cells, the principal cells of the dentate gyrus, Lire found to be resistant to damage in epilepsy. and may serve as generators of seizures if their inhibition is impaired. Therefore, the parvalbumin-containing subset of interneurons, known to provide the most powerful inhibitory input to granule cell somata and axon initial segments, were examined in human control and epileptic dentate gyrus. A strong reduction in the number of parvalbumin-containing cells was found in the epileptic samples especially in the hilar region, although in some patches of the granule cell layer parvalbumin-positive terminals that form vertical clusters characteristic of axoaxonic cells were more numerous than in controls. Analysis of the postsynaptic target elements of parvalbumin-positive axon terminals showed that they form symmetric synapses with somata, dendrites, axon initial segments and spines as in the control, but the ratio of axon initial segment synapses was increased in the epileptic tissue (control: 15.9%, epileptic: 31.3%). Furthermore. the synaptic coverage of granule cell axon initial segments increased more than three times (control: 0.52, epileptic: 2.10 mum synaptic length/100 mum axon initial segment membrane) in the epileptic samples, whereas the amount of somatic symmetric synapses did not change significantly. Although the number of parvalbumin-positive interneurons is decreased, the perisomatic inhibitory input of dentate granule cells is preserved in temporal lobe epilepsy. Basket and axo-axonic cell terminals - whether positive or negative for parvalbumin - are present, moreover, the axon collaterals targeting axon initial segments sprout in the epileptic dentate gyrus.We suggest that perisomatic inhibitory interneurons survive in epilepsy, but their somadendritic compartment and partly the axon loses parvalbumin or immunoreactivity for parvalbumin. The hyperinnervation of axon initial segments might be a compensatory change in the inhibitory network, but at the same time may lead to a more effective synchronization of granule cell firing that could contribute to the generation or amplification of epileptic seizures. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.