Infection with Specific Helicobacter pylori-cag Pathogenicity Island Strains Is Associated with Interleukin-1B Gene Polymorphisms in Venezuelan Chronic Gastritis Patients
Infection with Specific Helicobacter pylori-cag Pathogenicity Island Strains Is Associated with Interleukin-1B Gene Polymorphisms in Venezuelan Chronic Gastritis Patients
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DOI：
10.1007/s10620-010-1316-0
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发表时间：
2011-02-01
影响因子：
3.1
通讯作者：
Armanie, Emma
中科院分区：
文献类型：
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作者：
Angel Chiurillo, Miguel;Moran, Yeinmy H.;Armanie, Emma
CAG致病岛(CAG-PAI)是幽门螺杆菌的主要毒力因子之一，具有较大的地域差异性。我们调查了委内瑞拉胃癌高发区慢性胃炎患者CAG-PAI的cagA、cagE和cagT基因的分布及其与IL-1B-511/-31/+3954基因多态性的关系。采用聚合酶链式反应(PCR)和限制性片段长度多态性(RFLP)方法检测了121例活检标本中CAG-PAI基因和IL-1B基因的多态性。CagA(+)和cagAET(+)菌株分别占79.3%和70.2%。我们发现cagA(+)和cagAET(+)株的感染与同时携带IL-1B+3954C等位基因和IL-1B-511T/-31C/+3954C单倍型(TCC(+))的宿主相关(P<；0.05)。CagAET(+)/IL-1B-tcc(+)联合携带者胃萎缩发生率明显增高(P<0.020)，携带IL-1B+3954C等位基因和IL-1B-tcc(+)单倍型有利于细菌cagAET(+)的定植，这些细菌和宿主单倍型的结合在癌前病变的发生中可能起协同作用。这项工作有助于理解幽门螺杆菌毒力因子与胃肠道疾病所涉及的细胞因子基因之间的复杂相互作用。
The cag pathogenicity island (cag-PAI) is one of the major virulence factors of Helicobacter pylori, showing considerable geographic variation.We investigated the prevalence of cagA, cagE, and cagT genes of cag-PAI and their association with proinflammatory IL-1B-511/-31/+3954 polymorphisms in Venezuelan chronic gastritis patients from a high-risk gastric cancer region.Presence of cag-PAI genes and IL-1B polymorphisms in 121 biopsy specimens was evaluated by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP), respectively.cagA (+) and triple-positive (cagAET (+)) strains were detected in 79.3% and 70.2% of patients, respectively. We found that infection with cagA (+) and cagAET (+) strains was associated (P < 0.05) with hosts harboring both IL-1B +3954C allele and IL-1B-511T/-31C/+3954C haplotype (TCC (+)). The frequency of gastric atrophy was significantly higher (P < 0.020) among cagAET (+)/IL-1B-TCC (+) combined genotype carriers.Carriage of IL-1B +3954C allele and IL-1B-TCC (+) haplotype could favor colonization of bacterial cagAET (+) strains, and the combination of these bacterial and host haplotypes could play a synergistic role in development of premalignant gastric lesions. This work contributes to understanding of the complex interaction between H. pylori virulence factors and cytokine genotypes involved in gastrointestinal diseases.