DISTRIBUTION OF CORTICOTROPIN-RELEASING FACTOR-RECEPTOR MESSENGER-RNA EXPRESSION IN THE RAT-BRAIN AND PITUITARY
DISTRIBUTION OF CORTICOTROPIN-RELEASING FACTOR-RECEPTOR MESSENGER-RNA EXPRESSION IN THE RAT-BRAIN AND PITUITARY
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DOI：
10.1073/pnas.91.19.8777
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发表时间：
1994-09-13
影响因子：
11.1
通讯作者：
VALE, W
中科院分区：
文献类型：
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作者：
POTTER, E;SUTTON, S;VALE, W
促肾上腺皮质激素释放因子（Corticotropin-releasing factor，CRF）是调节垂体-肾上腺对应激反应的一种重要促垂体激素，在中枢神经系统广泛表达。最近克隆的编码人和大鼠CRF受体的cDNA使我们能够通过原位杂交绘制表达CRF受体mRNA的细胞在大鼠脑和垂体中的分布。前脑中的受体表达由遍及新皮质、嗅觉皮质和海马皮质的所有区域的广泛信号支配。CRF受体mRNA表达的其他突出部位包括隔区和杏仁核的皮质下边缘结构。在间脑中，在少数离散的丘脑腹侧核和下丘脑内侧核中观察到低水平的表达。CRF受体在下丘脑神经分泌结构，包括室旁核和正中隆起，表达普遍较低。在脑干中，某些中继核与躯体（包括三叉神经），听觉，前庭，内脏感觉系统，构成CRF受体mRNA表达的突出网站。此外，高水平的这种转录本存在于小脑皮质和深层核，沿着许多小脑前核。在垂体中，CRF受体mRNA的表达在整个中叶和前叶的细胞亚群中检测到中等水平，通过并行免疫标记确定为促皮质激素。总的来说，CRF受体mRNA表达的中心分布与报告的结合CRF的区域相似，但比其更广泛，并且与表达CRF结合蛋白的位点重叠有限。有趣的是，CRF受体mRNA是低的或检测不到的几个细胞群牵连作为CRF行动的中心网站。
Corticotropin-releasing factor (CRF) is a major hypophysiotropic peptide regulating pituitary-adrenal response to stress, and it is also widely expressed in the central nervous system. The recent cloning of cDNAs encoding the human ind rat CRF receptors has enabled us to map the distribution of cells expressing CRF receptor mRNA in rat brain and pituitary by in situ hybridization. Receptor expression in the forebrain is dominated by widespread signal throughout all areas of the neo-, olfactory, and hippocampal cortices. Other prominent sites of CRF receptor mRNA expression include subcortical limbic structures in the septal region and amygdala. In the diencephalon, low levels of expression are seen in a few discrete ventral thalamic and medial hypothalamic nuclei. CRF receptor expression in hypothalamic neurosecretory structures, including the paraventricular nucleus and median eminence, is generally low. In the brainstem, certain relay nuclei associated with the somatic (including trigeminal), auditory, vestibular, and visceral sensory systems, constituted prominent sites of CRF receptor mRNA expression. In addition, high levels of this transcript are present in the cerebellar cortex and deep nuclei, along with many precerebellar nuclei. In the pituitary, moderate levels of CRF receptor mRNA expression were detected throughout the intermediate lobe and in a subset of cells in the anterior lobe identified as corticotropes by concurrent immunolabeling. Overall, the central distribution of CRF receptor mRNA expression is similar to, though more expansive than, that of regions reported to bind CRF, and it shows limited overlap with loci expressing CRF-binding protein. Interestingly, CRF receptor mRNA is low or undetectable in several cell groups implicated as central sites of CRF action.