A simplified procedure for the quantitative measurement of neurological deficits after forebrain ischemia in mice
A simplified procedure for the quantitative measurement of neurological deficits after forebrain ischemia in mice
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DOI：
10.1016/j.jneumeth.2005.02.013
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发表时间：
2005-08-30
影响因子：
3
通讯作者：
San-Juan, ER
中科院分区：
文献类型：
--
作者：
Rodriguez, R;Santiago-Mejia, J;San-Juan, ER
本研究描述了一种全面的方法来评估脑缺血后的神经功能缺损所产生的连续颈总动脉切片（SCAS）在老年小鼠，和规模，以确定缺血动物的功能丧失的程度。该方法包括一个初始阶段的不受干扰的观察和后期的操作阶段，在此期间，每只动物都受到一系列非常简单的操作。假手术动物在整个研究期间（72 h）的存活率为96%，而SCAS小鼠的24、48和72 h存活率分别为48、38和36%。在存活的SCAS小鼠中，我们在整个观察期（72小时）共检测到23种神经系统变化;最常见的变化是：运动不协调、异常体位、运动能力低下、身体张力和肌肉力量下降、震颤、驼背、被动、前肢屈曲和共济失调步态。基于这些变化，我们使用了一个全球量表，其中包括10个超过0（正常）的渐进等级，扩展到状态10（由于SCAS死亡），分数越高表明缺陷越大。假手术动物在24、48和72 h的中位神经系统评分分别为1.36、1.48和1.32，而SCAS小鼠在24、48和72 h的总神经系统评分分别为6.1、6.8和7.4，显著高于假手术动物中观察到的评分。本文所述的测量缺血性动物的功能性神经状况的程序的简单性以及由SCAS产生的显著水平的功能损伤提供了测试推定的中风治疗的功效和监测动物组中随时间推移的缺陷进展的可能性。(c)2005 Elsevier B. V.保留所有权利。
This study describes a comprehensive method to assess neurological deficits after brain ischemia produced by sequential common carotid artery sectioning (SCAS) in aged mice, and a scale to determine the degree of functional incapacity of ischemic animals. The method involves an initial phase of undisturbed observation and a later manipulative phase during which each animal is subjected to a sequence of very simple manipulations. Sham-operated animals demonstrated 96% survival throughout the study period (72 h), whereas the 24, 48 and 72 h survival rates of SCAS-mice were 48, 38 and 36%, respectively. In the surviving SCAS-mice, we detected a total of 23 neurological alterations throughout the observation period (72 h); the most frequent alterations were: motor incoordination, abnormal body position, hypomobility, decreased body tone and muscular strength, tremor, hunched back, passivity, forelimb flexion and ataxic gait. Based on these alterations, we used a global scale that comprises 10 progressive grades beyond 0 (normal), extending to status 10 (death due to SCAS), with higher scores indicating greater deficit. The median neurological scores for sham-operated animals were 1.36, 1.48 and 1.32 at 24, 48 and 72 h, respectively, whereas total neurological scores in SCAS-mice of 6.1, 6.8 and 7.4, at 24, 48 and 72 h, respectively, were substantially greater than those observed in sham-operated animals. The simplicity of the procedure, herein described, to measure the functional neurological condition of ischemic animals, and the remarkable level of functional impairment produced by SCAS offer the possiblity to test the efficacy of putative stroke therapies and to monitor progress of deficits over time in groups of animals. (c) 2005 Elsevier B.V. All rights reserved.