Subcellular distribution of succinate-semialdehyde dehydrogenase in rat brain.
Subcellular distribution of succinate-semialdehyde dehydrogenase in rat brain.
复制标题
大鼠脑中琥珀酸半醛脱氢酶的亚细胞分布。
DOI：
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
G. A. Davis
中科院分区：
文献类型：
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作者：
K. Sims;G. A. Davis
报道了琥珀酸半醛脱氢酶在大鼠脑中的亚细胞定位。该酶在4-氨基丁酸分解代谢和脑中间能量代谢中起重要作用，并与谷氨酸脱羧酶和乳酸脱氢酶进行了定位比较。在蔗糖梯度亚组分中观察到琥珀半醛脱氢酶活性的独特显着过度恢复，这种过度恢复可以通过重复冷冻解冻或Triton处理来消除。Triton治疗也显示突触体部分有大量的这种活性，在标准程序中不明显，但活性的主要富集在线粒体部分。结果表明，脑内4-氨基丁酸酯合成反应的细胞定位与以琥珀酸半醛脱氢酶为代表的分解代谢系统存在显著的分离。
The report details the subcellular localization of succinate semialdehyde dehydrogenase in rat brain. The localization of this enzyme, which is important in the catabolism of 4-aminobutyrate and brain intermediary energy metabolism, is compared with glutamic acid decarboxylase and lactate dehydrogenase. A unique significant over-recovery of succinic semialdehyde dehydrogenase activity was observed in sucrose gradient subfractions, an over-recovery which could be eliminated by repeated freeze-thaws or Triton treatment of fractions. Triton treatment also revealed a significant amount of this activity in the synaptosomal fraction not apparent in the standard procedure, but the major enrichment of activity was found in the mitochondrial fraction. Our results indicate that a striking separation exists in the cellular localization of the synthetic reaction for 4-aminobutyrate in brain and the catabolic system represented by succinate semialdehyde dehydrogenase.