Studies on the metabolism of adipose tissue; some in vitro effects of a prolactin preparation alone and in combination with insulin or adrenalin.
Studies on the metabolism of adipose tissue; some in vitro effects of a prolactin preparation alone and in combination with insulin or adrenalin.
复制标题
脂肪组织代谢研究；
作者：
R. O. Moore;E. Ball
在体外测试了大鼠附睾脂肪组织在单独使用催乳素制剂（0.2-1.0 mg/ml）和与胰岛素或肾上腺素联合使用时的葡萄糖摄取、净气体交换、乳酸产生、耗氧量（在各种碳化合物存在的情况下）和游离脂肪酸释放。葡萄糖摄取和净气体交换被这种垂体制剂以一种模仿胰岛素作用的方式刺激。这两个过程都可以通过同时添加胰岛素进一步刺激，在净气体交换的情况下，效果是协同的。催乳素制剂减少组织乳酸的产生，并抵消胰岛素或肾上腺素刺激这种代谢物形成的作用。在葡萄糖存在的情况下，催乳素制剂引起氧气消耗的轻微但显著的增加。更引人注目的是，随着时间的推移，制备物能够稳定耗氧量的骤增。
The glucose uptake, net gas exchange, lactic acid production, oxygen consumption (in the presence of various carbon compounds) and free fatty acid release by rat epididymal adipose tissue in vitro have been measured in the presence of a prolactin preparation (0.2–1.0 mg/ml) alone and in combination with insulin or Adrenalin. Glucose uptake and net gas exchange are stimulated by this pituitary preparation in a manner that mimics the action of insulin. Both these processes may be stimulated further by the concomitant addition of insulin and, in the case of net gas exchange, the effect is synergistic. The prolactin preparation diminishes the production of lactic acid by the tissue and counteracts the action of insulin or Adrenalin in stimulating the formation of this metabolite. The prolactin preparation causes a slight but significant increase in oxygen consumption in the presence of glucose. More striking is the ability of the preparation to stabilize with time the burst in oxygen consumption seen upon add...