Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse.
Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse.
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DOI：
10.1016/j.biortech.2020.124499
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发表时间：
2020-12
影响因子：
11.4
通讯作者：
Thatiane R. Mota;Dyoni M. Oliveira;Rachael Simister;C. Whitehead;A. Lanot;W. D. dos Santos;C. A. Rezende;S. McQueen-Mason;L. Gomez
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文献类型：
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作者：
Thatiane R. Mota;Dyoni M. Oliveira;Rachael Simister;C. Whitehead;A. Lanot;W. D. dos Santos;C. A. Rezende;S. McQueen-Mason;L. Gomez
为了最大化甘蔗渣的糖释放，将高分辨率部分因子设计（FFD）与中心组合正交设计（CCO）相结合，以同时评估碱预处理的各种变量（NaOH：0.1-lmol/L，温度：100-220 °C，时间：20-80 min）和酶糖化（酶负载：2.5- 17.5%，反应体积：550-850 μL）。对46个实验条件进行了评价，在优化条件下（0.25 mol/L NaOH，202 °C，40 min，加酶量为12.5%），酶解18 h，糖得率最高（423 mg/g）。生物质组成分析表明，预处理强烈去除木质素（高达70%），二氧化硅（高达80%），并促进纤维素富集（25-110%）。这种稳健的实验设计导致甘蔗渣的酶水解效率最大化，并且进一步表明这种组合方法对于其他木质纤维素生物质是通用的。
To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1–1 mol/L, temperature: 100–220 °C, and time: 20–80 min) and enzymatic saccharification (enzyme loading: 2.5–17.5%, and reaction volume: 550–850 µL). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25–110%). This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.