Functional Implications of CD34 Expression in Human Adipose-Derived Stem/Progenitor Cells
Functional Implications of CD34 Expression in Human Adipose-Derived Stem/Progenitor Cells
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DOI：
10.1089/scd.2009.0003
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发表时间：
2009-10-01
影响因子：
4
通讯作者：
Yoshimura, Kotaro
中科院分区：
文献类型：
--
作者：
Suga, Hirotaka;Matsumoto, Daisuke;Yoshimura, Kotaro
CD 34常用作脂肪源性干/基质/祖细胞（ASC）的标志物。然而，CD 34在人ASC（hASC）中的表达随着培养时间的推移而降低，并且其含义仍不清楚。在这项研究中，我们将短期培养的hASC分为CD 34+和CD 34-部分，并比较它们的生物学功能。结果表明，CD 34 + hASCs更具增殖性，并具有更强的集落形成能力。相反，CD 34-细胞表现出更大的分化成脂肪和成骨谱系的能力。CD 34+和CD 34-细胞对生长因子的迁移和毛细血管形成能力相似。内皮祖细胞标志物（Flk-1、FLT 1和Tie-2）的表达水平在CD 34+细胞中较高，而周细胞标志物（CD 146、NG 2和α-平滑肌肌动蛋白）的表达水平在CD 34-细胞中较高。CD 34+和CD 34-细胞显示相似的血管内皮生长因子和肝细胞生长因子的表达水平，虽然缺氧影响表达水平。总之，这些结果表明，CD 34在hASCs的表达可能与复制能力，分化潜能，血管生成相关基因的表达谱，和不成熟或干细胞。CD 34表达的丧失可能与从未成熟状态定型和/或分化为特定谱系（如脂肪、骨或平滑肌）的生理过程有关。
CD34 is frequently used as a marker of adipose-derived stem/stromal/progenitor cells (ASCs). However, CD34 expression in human ASCs (hASCs) decreases over time in culture, and the implications of this remain unclear. In this study, we sorted shortly-cultured hASCs into CD34+ and CD34- fractions and compared their biological functions. Results indicated that CD34+ hASCs were more proliferative and had a greater ability to form colonies. In contrast, CD34-cells showed a greater ability for differentiation into adipogenic and osteogenic lineages. Both CD34+ and CD34-cells showed similar abilities in migration and capillary formation in response to growth factors. Expression levels of endothelial progenitor markers (Flk-1, FLT1, and Tie-2) were higher in CD34+ cells, whereas those of pericyte markers (CD146, NG2, and alpha-smooth muscle actin) were higher in CD34- cells. Both CD34+ and CD34-cells showed similar expression levels of vascular endothelial growth factor and hepatocyte growth factor, although hypoxia affected the expression levels. Together these results suggest that CD34 expression in hASCs may correlate with replicative capacity, differentiation potentials, expression profiles of angiogenesis-related genes, and immaturity or stemness of the cells. Loss of CD34 expression may be related to the physiological process of commitment and/or differentiation from immature status into specific lineages such as adipose, bone, or smooth muscle.