Osteoblastic cell behaviour on different titanium implant surfaces
Osteoblastic cell behaviour on different titanium implant surfaces
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DOI：
10.1016/j.actbio.2007.12.002
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发表时间：
2008-05-01
影响因子：
9.7
通讯作者：
Layrolle, Pierre
中科院分区：
文献类型：
--
作者：
Le Guehennec, Laurent;Lopez-Heredia, Marco-Antonio;Layrolle, Pierre
口腔种植体的骨整合与成骨细胞与钛表面的早期相互作用有关。我们比较了成骨细胞MC3T3-E1在四种不同钛表面上的行为：镜面抛光（Smooth-Ti）、氧化铝喷砂（aluminum - ti）或双相磷酸钙陶瓷喷砂（BCP-Ti）和市售种植体表面（SLA）。扫描电镜和轮廓术显示明显的显微形貌。BCP-Ti组的平均表面粗糙度（R-a = 2.5 μ m）高于其他喷砂组。通过动态接触角测量测定了不同基质上的亲水性和表面能。最亲水的表面是氧化铝-钛盘，而最疏水的表面是SLA。通过x射线光电子能谱测定，钛表面均被氧化为TiO2，并被碳污染物污染。在爆破作用下，铝钛样品也出现铝峰。BCP-Ti圆盘含有微量的钙和磷。MC3T3-E1细胞在底物上附着、扩散和增殖。对于SLA和BCP-Ti组，2天后整个表面都覆盖了一层成骨细胞。在高倍镜下，细胞表现出细胞质延伸和丝状足。与塑料组相比，细胞活力与Smooth-Ti组相似，与Alumina-Ti组略低，与SLA和BCP-Ti组较好。碱性磷酸酶活性随培养时间的增加而增加。本研究表明，bcp爆破产生的粗钛种植体表面没有污染物。(c) 2007材料学报Elsevier Ltd.出版。版权所有。
The osseointegration of oral implants is related to the early interactions between osteoblastic cells and titanium surfaces. The behaviour of osteoblastic MC3T3-E1 cells was compared on four different titanium surfaces: mirror-polished (Smooth-Ti), alumina grit-blasted (Alumina-Ti) or biphasic calcium phosphate ceramic grit-blasted (BCP-Ti) and a commercially available implant surface (SLA). Scanning electron microscopy and profilometry showed distinct microtopographies. The BCP-Ti group had higher average surface roughness (R-a = 2.5 mu m) than the other grit-blasted groups. Hydrophilicity and surfaces energies were determined on the different substrates by dynamic contact angle measurements. The most hydrophilic surface was the Alumina-Ti discs, while SLA was the most hydrophobic. The titanium surfaces were all oxidized as TiO2 and polluted by carbon contaminants, as determined by X-ray photoelectron spectroscopy. Alumina-Ti samples also exhibited aluminium peaks as a result of the blasting. The BCP-Ti discs contained traces of calcium and phosphorus. MC3T3-E1 cells attached, spread and proliferated on the substrates. For both the SLA and BCP-Ti groups, the entire surface was covered with a layer of osteoblastic cells after 2 days. At high magnification, the cells exhibited cytoplasmic extensions and filopodia. Compared with plastic, cell viability was similar with the Smooth-Ti, slightly lower with the Alumina-Ti and superior with the SLA and BCP-Ti groups. Alkaline phosphatase activity increased with the culture time whatever the substrate. This study shows that BCP-blasting produces rough titanium implants without surface contaminants. (c) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.