LENS DEVELOPMENT .I. ROLE OF LENS IN EYE GROWTH
LENS DEVELOPMENT .I. ROLE OF LENS IN EYE GROWTH
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DOI：
10.1002/jez.1401560104
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发表时间：
1964-01-01
影响因子：
--
通讯作者：
COULOMBRE, JL
中科院分区：
文献类型：
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作者：
COULOMBRE, AJ;COULOMBRE, JL
以3种方式对孵育5天的鸡胚的眼睛进行手术：a）通过边缘切口取出透镜，随后愈合; B）从眼睛中取出透镜并立即更换; c）从眼睛中取出透镜并更换为煮沸15分钟的5天透镜。在这些程序之后，测量眼睛直径、角膜直径和玻璃体重量作为时间的函数。在没有透镜的情况下，或在存在煮沸的透镜的情况下，眼睛的生长显著降低，并且玻璃体物质不能积聚。摘除透镜后立即更换，导致眼睛正常生长。结果解释如下：a）神经视网膜的生长和分化不依赖于透镜; B）角膜的生长和分化依赖于活体透镜，直到至少孵育第5天。如果除去透镜，角膜逐渐被其它组织取代; c）色素上皮、脉络膜和巩膜的正常生长和形状变化需要透镜的存在。透镜对这3层的影响是通过其对玻璃体物质在玻璃体腔中积聚的控制来介导的。
The eyes of chick embryos at 5 days of incubation were operated on in 3 ways: a) the lens was removed through a limbic incision which subsequently healed; b) the lens was removed from the eye and immediately replaced; c) the lens was removed from the eye and replaced by a 5-day lens which had been boiled for 15 minutes. Eye diameter, corneal diameter, and vitreous weight were measured as a function of time following these procedures. In the absence of the lens, or in the presence of a boiled lens, the growth of the eye was markedly reduced, and vitreous substance failed to accumulate. Removal of the lens followed by its immediate replacement resulted in normal growth of the eye. The results are interpreted as follows: a) the neural retina grows and differentiates independently of the lens; b) the cornea is dependent upon the living lens for its growth and differentiation until at least the 5th day of incubation. The cornea is gradually replaced by other tissues if the lens if removed; c) the normal growth and change in shape of the pigmented epithelium, choroid coat and sclera require the presence of the lens. The influence of the lens on these 3 coats is mediated by its control of the accumulation of vitreous substance in the vitreous chamber.