MODULATION BY APPLIED ELECTRIC-FIELDS OF PURKINJE AND STELLATE CELL-ACTIVITY IN THE ISOLATED TURTLE CEREBELLUM
MODULATION BY APPLIED ELECTRIC-FIELDS OF PURKINJE AND STELLATE CELL-ACTIVITY IN THE ISOLATED TURTLE CEREBELLUM
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DOI：
10.1113/jphysiol.1986.sp015963
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发表时间：
1986-02-01
影响因子：
5.5
通讯作者：
NICHOLSON, C
中科院分区：
文献类型：
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作者：
CHAN, CY;NICHOLSON, C
1.在离体乌龟小脑上施加准稳态电场，研究外加电场与神经元形态和神经元放电模式调制之间的关系。2.使用细胞外记录方法和随后的辣根过氧化物酶注射，这揭示了它们的树突状形态和方向的电生理确定的尖峰元素。3.通过测量40 s恒流脉冲在小脑中诱导的电压梯度来精确定义电场。场在垂直（背腹）轴上恒定，在水平面上为零，与理论一致。4.通过施加频率在0.05和1.0 Hz之间的正弦场来调制神经元。受调制的细胞表现出放电频率的增加，并根据产生调制的场的方向分为四类之一。因此，神经元被以下因素兴奋：心室定向场（V调制）、成对定向场（P调制）、上述两者（V/P调制）或未显示一致的调制（非调制）。5.大多数浦肯野胞体和初级树突（28个中的19个）和大多数浦肯野树突（30个中的18个）以与峰值场强成比例的最大速率进行V调制。这些细胞的树突一致地朝向软脑膜。6.在星状细胞中，低分子层星状细胞，树突主要向软脑膜延伸，大多数（32个中的19个）V调制。中分子层的星形，这表明在树枝状取向的变化很大，分布在所有四个调制类。上分子层的恒星，主要是水平的树枝状排列，主要是（十六分之九）非调制。7.所有组的尖峰元素表现出的调制模式之间的相关性，由外加磁场和树突的方向，这表明的程度的差异极化的延伸电缆元件的神经元的外加磁场的基本机制，为场诱导的兴奋或抑制。8.所有神经元的调制阈值为15-20 mV/mm，这与调制其他神经组织的场相似。这表明许多神经元可以被10-20 mV/mm量级的场调制。
1. Quasi steady-state electric fields were applied across the isolated turtle cerebellum to study the relationship between applied field, neuronal morphology and the modulation of the neuronal spike firing pattern. 2. Spiking elements were identified electrophysiologically using extracellular recording methods and by subsequent horseradish peroxidase injection, which revealed their dendritic morphology and orientation. 3. The electric field was precisely defined by measuring the voltage gradients induced in the cerebellum by 40 s constant-current pulses. The field was constant in the vertical (dorso-ventral) axis and zero in the horizontal plane, in agreement with theory. 4. Neurons were modulated by applying a sinusoidal field at frequencies between 0.05 and 1.0 Hz. Modulated cells exhibited an increase in firing frequency and fell into one of four classes, depending on the direction of the field that produced the modulation. Thus neurons were excited by: ventricle-directed fields (V modulation), pair-directed fields (P modulation), both of the above (V/P modulation) or showed no consistent modulation (non-modulation). 5. Most Purkinje somata and primary dendrites (nineteen out of twenty-eigth) and most Purkinje dendrites (eighteen out of thirty), were V modulated with maximum rate proportional to the peak field intensity. The dendrites of these cells were consistently oriented toward the pia. 6. Among the stellate cells, the lower molecular layer stellates, with dendrites extending predominantly towards the pia, were mostly (nineteen out of thirty-two) V modulated. The mid-molecular layer stellates, which showed much variability in dendritic orientation, were distributed among all four of the modulation classes. The upper molecular layer stellates, with mostly horizontal dendritic alignment, were mainly (nine out of sixteen) non-modulated. 7. All groups of spiking elements showed a correlation between patterns of modulation by applied fields and dendritic orientation, which suggest the degree of differential polarization of the extended cable elements of the neurone by the applied field as the basic mechanism for field-induced excitation or inhibition. 8. The threshold for modulation among all neurones was 15-20 mV/mm, which is similar to the fields that modulate other nervous tissues. This suggests that many neurons can be modulated by fields of the order of 10-20 mV/mm.