IMMUNOCYTOCHEMICAL STUDY ON THE IMMUNE COMPLEX AND GERMINAL CENTER OF SYNOVIAL TISSUE OF RHEUMATOID ARTHRITIS WITH SPECIAL REGARD TO COMPLEMENT AND FOLLICULAR DENDRITIC CELLS
IMMUNOCYTOCHEMICAL STUDY ON THE IMMUNE COMPLEX AND GERMINAL CENTER OF SYNOVIAL TISSUE OF RHEUMATOID ARTHRITIS WITH SPECIAL REGARD TO COMPLEMENT AND FOLLICULAR DENDRITIC CELLS
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类风湿性关节炎滑膜组织免疫复合物和生发中心的免疫细胞化学研究，特别关注补体和滤泡树突状细胞
DOI：
10.1111/j.1440-1827.1986.tb03118.x
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发表时间：
1986
影响因子：
2.2
通讯作者：
Y. Imai
中科院分区：
文献类型：
--
作者：
T. Kasajima;Takashi Sato;M. Yamakawa;Akihiko Masuda;Y. Imai
为了分析类风湿性关节炎（RA）滑膜组织中淋巴生发中心和滤泡树突状细胞（FDC）的免疫学作用，我们尝试通过光镜和电镜免疫细胞化学检测生发中心的免疫复合物，特别强调免疫球蛋白、补体成分、RA因子和DRC - 1抗原。免疫球蛋白主要分布在生发中心的细胞间，呈网状分布，在一些生发中心淋巴样细胞中部分分布在胞浆内。经典途径早期补体成分（C1q、C4、C3c和C3d）和RA因子的分布与免疫球蛋白相似，但从未观察到胞浆内阳性。这些共存的正电荷位点与FDC膜扩展过程表面的DRC - 1正电荷位点相同。与生发中心相比，在初级卵泡或淋巴样聚集体中观察到类似的发现较少。这些结果表明，一些生发中心捕获了免疫复合物，其中RA因子至少与fdc密切相关，RA因子是抗原和发育中的生发中心的触发因素之一。
To analyze the immunological role of Iymphoid germinal centers and follicular dendritic cells (FDC) in synovial tissue of rheumatoid arthritis (RA), we tried to detect the immune complex in germinal centers by light and electron microscopic immunocytochemistry with special emphasis on immunoglobulin, complement components, RA factor, and DRC‐1 antigen. Immunoglobulins mainly distributed intercellularly in the germinal center in a lacy network pattern, and show partial intracytoplasmic localization in some germinal center lymphoid cells. Early complement components of classical pathway (C1q, C4, C3c, and C3d) and RA factor distributed lacily similarily to immunoglobulin, but intracytoplasmic positivity is never observed. These coexistent positive sites are identical to DRC‐1 positive sites which are the surface of extended processes of FDC membrane. A similar finding is observed in primary follicles or lymphoid aggregates less often than germinal centers. These results indicate that some germinal centers trap the immune complex, including RA factor at least closely related with FDCs, and also RA factor is one of the triggers of antigen as well as developing germinal centers.