Sulphur-oxidizing and sulphate-reducing communities in Brazilian mangrove sediments.
Sulphur-oxidizing and sulphate-reducing communities in Brazilian mangrove sediments.
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DOI：
10.1111/1462-2920.12237
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发表时间：
2014-03
影响因子：
5.1
通讯作者：
Maryeimy Varón-López;A. C. F. Dias;C. C. Fasanella-C.;Ademir Durrer;I. S. Melo;E. Kuramae;F. D. Andreote
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文献类型：
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作者：
Maryeimy Varón-López;A. C. F. Dias;C. C. Fasanella-C.;Ademir Durrer;I. S. Melo;E. Kuramae;F. D. Andreote
红树林土壤是富含硫酸盐和有机质的厌氧环境。尽管硫循环是这一生态系统中的主要因素之一，但人们对红树林土壤中的硫细菌群落知之甚少。调查了巴西三个红树林群落中硫氧化(SOB)和硫酸盐还原(SRB)细菌的丰度、组成和多样性，其中两个被污染，一个被石油污染(OilMgv)，一个被城市垃圾和污泥污染(AntMgv)，以及一个原始(PrsMgv)。利用实时定量聚合酶链式反应(QPCR)、聚合酶链式反应-变性梯度凝胶电泳法(PCR-DGGE)和克隆文库，利用腺苷-5‘-磷酸还原酶(APRA)和亚硫酸盐还原酶(DSR)(DsrB)基因对群落结构进行了评价。定量聚合酶链式反应的丰度表明，dsrB/apra的比值在不同的红树林中是不同的，并且随着观测到的石油污染的梯度而较高。非度量多维尺度分析的PCR-DGGE图谱揭示了三种红树群落结构的差异。克隆文库显示，与红树林沉积物中硫循环相关的类群最丰富的是β蛋白细菌、伽马蛋白细菌和三角洲蛋白细菌。因此，红树林土壤中的微生物SOB和SRB群落在不同的红树林中是不同的，这些微生物群落可能可以作为红树林污染的替代指标。
Mangrove soils are anaerobic environments rich in sulphate and organic matter. Although the sulphur cycle is one of the major actors in this ecosystem, little is known regarding the sulphur bacteria communities in mangrove soils. We investigated the abundance, composition and diversity of sulphur-oxidizing (SOB) and sulphate-reducing (SRB) bacteria in sediments from three Brazilian mangrove communities: two contaminated, one with oil (OilMgv) and one with urban waste and sludge (AntMgv), and one pristine (PrsMgv). The community structures were assessed using quantitative real-time polymerase chain reaction (qPCR), polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and clone libraries, using genes for the enzymes adenosine-5'-phosphosulphate reductase (aprA) and sulphite reductase (Dsr) (dsrB). The abundance for qPCR showed the ratio dsrB/aprA to be variable among mangroves and higher according to the gradient observed for oil contamination in the OilMgv. The PCR-DGGE patterns analysed by Nonmetric Multidimensional Scaling revealed differences among the structures of the three mangrove communities. The clone libraries showed that Betaproteobacteria, Gammaproteobacteria and Deltaproteobacteria were the most abundant groups associated with sulphur cycling in mangrove sediments. We conclude that the microbial SOB and SRB communities in mangrove soils are different in each mangrove forest and that such microbial communities could possibly be used as a proxy for contamination in mangrove forests.