Live Imaging of Organelle Motility in RPE Flatmounts
Live Imaging of Organelle Motility in RPE Flatmounts
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DOI：
10.1007/978-3-030-27378-1_64
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Williams, David S.
中科院分区：
文献类型：
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作者：
Umapathy, Ankita;Williams, David S.
视网膜色素上皮(RPE)具有多种对正常视网膜功能和视力至关重要的功能，包括每日吞噬感光细胞外节(POS)膜。POS吞噬小体的运动和降解缺陷可能与一些遗传性和年龄相关的视网膜变性有关。鉴于吞噬小体在成熟和降解过程中从顶端到底端的移位，对潜在机制的研究需要对三维动力学进行分析。在这一章中，我们报告了一种利用高速旋转圆盘共聚焦显微镜对活体RPE平板进行POS吞噬小体和溶酶体三维运动性研究的方法。
The retinal pigment epithelium (RPE) performs several functions that are crucial for normal retinal function and vision, including the daily phagocytosis of photoreceptor outer segment (POS) membranes. Defects in the motility and degradation of POS phagosomes may be associated with some inherited and age-related retinal degenerations. Given the apical to basal translocation of phagosomes during maturation and degradation, studies of the underlying mechanisms require analyses of the dynamics in 3-D. In this chapter, we report a method for investigating the 3-D motility of POS phagosomes and lysosomes, utilizing high-speed, spinning disk confocal microscopy of live RPE flatmounts.