Chemokine expression in IBD. Mucosal chemokine expression is unselectively increased in both ulcerative colitis and Crohn's disease
Chemokine expression in IBD. Mucosal chemokine expression is unselectively increased in both ulcerative colitis and Crohn's disease
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DOI：
10.1002/path.1245
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发表时间：
2003-01-01
影响因子：
7.3
通讯作者：
Sheron, N
中科院分区：
文献类型：
--
作者：
Banks, C;Bateman, A;Sheron, N
炎症性肠病（IBD）中的粘液变化的特征是溃疡性病变，伴有肠壁中显著的细胞浸润。趋化因子是能够促进白细胞迁移到炎症区域并且还能够启动细胞活化事件的趋化性细胞因子。它们最近被牵连在许多疾病状态的病理生理学。本研究的目的是详细的程度和分布的特异性趋化因子，白细胞介素（IL）-8，单核细胞趋化蛋白（MCP）-1，-2和-3，巨噬细胞炎症蛋白（MIP）-1 α和-1 β，在IBD粘膜。包括39名患者，10名对照，20名溃疡性结肠炎（UC）和9名克罗恩病（CD），具有一系列疾病活动。从UC、CD和对照患者中收集结肠粘膜活检，并包埋在乙二醇甲基丙烯酸酯中。切下2微米厚的切片，并使用免疫组织化学染色以检测趋化因子蛋白表达。使用光学显微镜分析切片。所有类型的趋化因子蛋白的表达检测从对照组和IBD患者的结肠粘膜。IBD患者和对照组之间的染色模式显著不同，但CD和UC患者表现出相似的染色模式。个别趋化因子的表达被认为是显着上调炎症性肠病患者相比，非患病组在所有领域的粘膜切片。趋化因子表达上调与疾病活动增加相关。结论：人结肠趋化因子的表达在IBD中是非选择性上调的。结果支持了UC和CD中局部炎症和组织损伤的程度取决于IBD组织内特异性趋化因子的局部表达的假设。版权所有（C）2002约翰威利父子有限公司
Mucosal changes in inflammatory bowel disease (IBD) are characterized by ulcerative lesions accompanied by prominent cellular infiltrates in the bowel wall. Chemokines are chemotactic cytokines that are able to promote leukocyte migration to areas of inflammation and are also able to initiate cell activation events. They have recently been implicated in the pathophysiology of many disease states. The aim of this study was to detail the degree and distribution of specific chemokines, interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, -2, and -3, and macrophage inflammatory protein (MIP)-1alpha and -1beta, in IBD mucosa. Thirty-nine patients were included, ten controls, 20 ulcerative colitis (UC), and nine Crohn's disease (CD), with a range of disease activity. Colonic mucosal biopsies were collected from UC, CD, and control patients and embedded in glycol methacrylate. Two-micrometre-thick sections were cut and stained using immunohistochemistry for chemokine protein expression. Sections were analysed using a light microscope. Expression of all types of chemokine protein was detected in colonic mucosa from both control and IBD patients. Patterns of staining between IBD patients and controls differed significantly, but CD and UC patients demonstrated similar patterns of staining. Individual chemokine expression was found to be significantly up-regulated in IBD when patients were compared with the non-diseased group in all areas of the mucosal sections. Up-regulated chemokine expression correlated with increasing activity of the disease. It is concluded that human colonic chemokine expression is non-selectively up-regulated in IBD. The results supported the hypothesis that the degree of local inflammation and tissue damage in UC and CD is dependent on local expression of specific chemokines within IBD tissues. Copyright (C) 2002 John Wiley Sons, Ltd.