Facile synthesis of nitrogen-doped carbon dots and its application as sensing probes for serum iron
Facile synthesis of nitrogen-doped carbon dots and its application as sensing probes for serum iron
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DOI：
10.1007/s11051-015-3252-6
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发表时间：
2015-11
影响因子：
2.5
通讯作者：
Long Wang;Juan Hou;Huiyu Li;Qi Zhao;Fengshuang Zhang;Jiahui Zhao;H. Ding;L. Ding
中科院分区：
文献类型：
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作者：
Long Wang;Juan Hou;Huiyu Li;Qi Zhao;Fengshuang Zhang;Jiahui Zhao;H. Ding;L. Ding
以尿素为原料，二甘醇为高沸点反应介质，采用微波热解法成功制备了量子产率为23.6%的氮掺杂碳量子点（N-CD）。N-CD为球形且单分散，尺寸分布在1.5和5.5 nm之间。N-CD具有优异的水溶性，在极端离子强度和光照下具有显著的稳定性。Fe ~（3+）能通过静态猝灭机制猝灭N-CD的荧光，而其它常见金属离子对N-CD的荧光无猝灭作用。在此基础上，N-CD可以作为一个简单的传感平台，用于Fe 3+的无标记灵敏和选择性检测，线性范围为1.6-333.3 μmol L−1，检测限为0.45 μmol L− 1，信噪比为3。最重要的是，基于N-CD的荧光探针成功地应用于人血清样品中铁含量的直接分析，这在生物和临床分析中显示了潜在的应用。
In this paper, nitrogen-doped carbon dots (N-CDs) with a quantum yield of 23.6 % were successfully synthesized by microwave pyrolysis of urea using diethylene glycol as the high boiling point reaction medium. The N-CDs were spherical and monodisperse with a size distribution between 1.5 and 5.5 nm. The N-CDs exhibited excellent water-soluble property and remarkable stability under extreme ionic strengths and light illumination. The fluorescence of the N-CDs could be quenched by Fe3+through the static quenching mechanism, but not by other common metal ions. On this basis, the N-CDs can be used as a facile sensing platform for label-free sensitive and selective detection of Fe3+in a linear range of 1.6–333.3 μmol L−1, and the detection limit was 0.45 μmol L−1obtained at a signal-to-noise ratio of 3. Importantly, the N-CDs-based fluorescent probe was successfully applied to the direct analysis of iron contents in human serum samples, which demonstrated potential applications in biological and clinical analysis.