EFFECT OF CHLORINE SUBSTITUTION ON THE BACTERIAL METABOLISM OF VARIOUS POLYCHLORINATED BIPHENYLS
EFFECT OF CHLORINE SUBSTITUTION ON THE BACTERIAL METABOLISM OF VARIOUS POLYCHLORINATED BIPHENYLS
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DOI：
10.1128/aem.38.2.301-310.1979
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发表时间：
1979-01-01
影响因子：
4.4
通讯作者：
KAMIBAYASHI, A
中科院分区：
文献类型：
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作者：
FURUKAWA, K;TOMIZUKA, N;KAMIBAYASHI, A
在检测的 36 种多氯联苯纯异构体（氯数 1-5）中，有 23 种化合物被产碱菌代谢。菌株 Y42 和 33 种化合物由不动杆菌代谢。菌株 P6。所检测的许多多氯联苯异构体的主要途径被认为是通过2'',3''-二氢-2'',3''-二醇化合物，伴随脱氢的2'',3''-二羟基化合物，随后是1'',2''-间位裂解化合物（2-羟基-6-氧代-6-苯基六-2,4-二烯酸的氯化衍生物），然后氯苯甲酸。在许多多氯联苯中进一步孵育后，间位裂解产物通常会转化为氯苯甲酸，但它们在 2,4''-、2,4,4''- 和 2,5,4''- 氯联苯的代谢中特异性积累，这些氯联苯都在分子的 2,4''-位上进行氯化。二羟基化合物主要在不动杆菌属的2,6-2,3,6-、2,4,2'',5''-、2,5,2'',5''-和2,4,5,2'',5''-氯联苯代谢中积累。 P6。 2,3,2'',3''-、2,3,2'',5''-和2,4,5,2'',3''-氯联苯在其中一个环的2,3-位上被氯化，以不同的方式代谢。氯苯甲酸和未知化合物的两种主要代谢物总是在这组多氯联苯的代谢中积累。 2,4,6-三氯联苯在两种生物体之间的代谢差异很大。产碱菌属 sp. Y42 通过主要氧化途径将该化合物非常缓慢地代谢为三氯苯甲酸。相反，不动杆菌属。 P6通过二羟基化合物将其代谢为三羟基化合物。
Of 36 pure isomers (chlorine numbers 1-5) of polychlorinated biphenyls examined, 23 compounds were metabolized by Alcaligenes sp. strain Y42 and 33 compounds were metabolized by Acinetobacter sp. strain P6. The major pathway of many polychlorinated biphenyl isomers examined was considered to proceed through 2'',3''-dihydro-2'',3''-diol compounds, concomitant dehydrogenated 2'',3''-dihydroxy compounds, subsequently the 1'',2''-meta-cleavage compounds (chlorinated derivatives of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acids), and then chlorobenzoic acids. The meta-cleavage products were usually converted to chlorobenzoic acids upon further incubation in many polychlorinated biphenyls, but they accumulated specifically in the metabolism of 2,4''-, 2,4,4''-, and 2,5,4''- chlorobiphenyls, which are all chlorinated at the 2,4''-position in the molecules in common. Dihydroxy compounds accumulated mainly in the metabolism of 2,6-2,3,6-, 2,4,2'',5''-, 2,5,2'',5''-, and 2,4,5,2'',5''-chlorobiphenyls by Acinetobacter sp. P6. The 2,3,2'',3''-, 2,3,2'',5''- and 2,4,5,2'',3''-chlorobiphenyls, which are chlorinated at the 2,3-position of one of the rings, were metabolized in a different fashion. Two major metabolites of a chlorobenzoic acid and an unknown compound always accumulated in the metabolism of this group of polychlorinated biphenyls. 2,4,6-Trichlorobiphenyl was metabolized quite differently between the 2 organisms. Alcaligenes sp. Y42 metabolized this compound very slowly to trichlorobenzoic acid by the major oxidative route. In contrast, Acinetobacter sp. P6 metabolized it to a trihydroxy compound via a dihydroxy compound.