Phosphatidylserine externalization is a downstream event of interleukin-1 beta-converting enzyme family protease activation during apoptosis.
Phosphatidylserine externalization is a downstream event of interleukin-1 beta-converting enzyme family protease activation during apoptosis.
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磷脂酰丝氨酸外化是细胞凋亡过程中白细胞介素 1 β 转换酶家族蛋白酶激活的下游事件。
作者：
M. Naito;M. Naito;Kimie Nagashima;Kimie Nagashima;T. Mashima;T. Mashima;T. Tsuruo;T. Tsuruo
磷脂酰丝氨酸（PS）是一类酸性磷脂，通常位于细胞质膜的内表面。当细胞发生凋亡时，内部PS被外部化;然而，其机制在很大程度上是未知的。为了研究PS外化在细胞凋亡过程中的机制，我们研究了白细胞介素-1 β转换酶（ICE）家族蛋白酶的激活和PS外化在人单核细胞白血病U937细胞及其耐药突变体UK 711和UK 110中的相关性，用足叶乙甙和抗Fas抗体处理后。我们发现，PS外化伴随着这些细胞系的凋亡和ICE家族蛋白酶的激活的发展。此外，ICE家族蛋白酶的抑制剂，Z-Asp和Z-VAD，防止凋亡和PS externalization在依托泊苷处理的U937细胞。这些结果表明，PS外化是ICE家族蛋白酶激活的下游事件在细胞凋亡的发展。由于ICE家族蛋白酶在细胞凋亡中起重要作用，PS外化可能是细胞凋亡发生发展的一个合理和有用的标志物。
Phosphatidylserine (PS), a class of acidic phospholipids, normally localizes on the internal surface of cellular plasma membranes. The internal PS is externalized when cells undergo apoptosis; however, the mechanism for this is largely unknown. To study the mechanism of PS externalization during development of apoptosis, we examined the correlation between the activation of interleukin-1 beta-converting enzyme (ICE) family protease and PS externalization in human monocytic leukemia U937 cells and in their apoptosis-resistant variants, UK711 and UK110, after treatment with etoposide and anti-Fas antibody. We found that PS externalization accompanied the development of apoptosis and the activation of ICE family proteases in these cell lines. Furthermore, inhibitors of ICE family proteases, Z-Asp and Z-VAD, prevented apoptosis and PS externalization in etoposide-treated U937 cells. These results indicate that PS externalization is a downstream event of ICE family protease activation during apoptosis development. Because ICE family proteases play a crucial role in apoptosis, PS externalization could be a rational and useful marker for the development of apoptosis.
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DOI：
10.1016/s0021-9258(18)47344-9
发表时间：
1994-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Fernandes‐Alnemri;G. Litwack;E. Alnemri
通讯作者：
T. Fernandes‐Alnemri;G. Litwack;E. Alnemri
影响因子：
10.5
作者：
KUMAR, S;KINOSHITA, M;JENKINS, NA
通讯作者：
JENKINS, NA
将荧光磷脂酰丝氨酸插入红细胞的质膜中。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tanaka,Y;Schroit,AJ
通讯作者：
Schroit,AJ
Mch2，凋亡Ced-3/Ice半胱氨酸蛋白酶基因家族的新成员。
DOI：
--
发表时间：
1995
期刊：
Cancer research.
影响因子：
--
作者：
Fernandes-Alnemri,T;Litwack,G;Alnemri,ES
通讯作者：
Alnemri,ES