Nuclear receptor coactivator‐6 is essential for the morphological change of human uterine stromal cell decidualization via regulating actin fiber reorganization
Nuclear receptor coactivator‐6 is essential for the morphological change of human uterine stromal cell decidualization via regulating actin fiber reorganization
复制标题
核受体辅激活因子-6通过调节肌动蛋白纤维重组对人子宫基质细胞蜕膜化的形态变化至关重要
DOI：
10.1002/mrd.23568
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发表时间：
2022
影响因子：
2.5
通讯作者：
Nagase Satoru
中科院分区：
文献类型：
--
作者：
Watanabe Norikazu;Kawagoe Jun;Sugiyama Akiko;Takehara Isao;Ohta Tsuyoshi;Nagase Satoru
核受体辅激活因子6（Ncoa 6）是一种核受体和转录因子的调节因子，在小鼠子宫内膜间质细胞的蜕膜化过程中起重要作用。然而，Ncoa6在人类子宫内膜中的功能仍不清楚。我们研究了其在从切除的子宫中分离的人子宫内膜基质细胞（HESCs）的蜕膜化中的功能。使用两种独立的小干扰RNA进行Ncoa6的敲低。在体外通过甲羟孕酮和环磷酸腺苷诱导蜕膜化。我们比较了Ncoa6敲低细胞和对照细胞之间的蜕膜细胞形态。进行信使RNA（mRNA）测序以确定未蜕膜化HESC中的Ncoa6靶基因。我们发现Ncoa6的敲低导致蜕膜化HESCs的形态学变化与对照细胞相比失败。mRNA测序显示Ncoa6调节与肌动蛋白纤维调节相关的基因的表达。Ncoa6敲除细胞在HESC的蜕膜化过程中未能重组肌动蛋白纤维。Ncoa6被证明通过适当调节HESC中的肌动蛋白纤维调节在蜕膜化中发挥重要作用。在此，我们的体外研究揭示了子宫内膜蜕膜化的部分机制。未来的研究需要调查这些机制的妇女植入缺陷。
Nuclear receptor coactivator 6 (Ncoa6), a modulator of several nuclear receptors and transcription factors, is essential for the decidualization of endometrial stromal cells in mice. However, the function of Ncoa6 in the human endometrium remains unclear. We investigated its function in the decidualization of human endometrial stromal cells (HESCs) isolated from resected uteri. Knockdown of Ncoa6 was performed using two independent small interfering RNAs. Decidualization was induced in vitro via medroxyprogesterone and cyclic adenosine monophosphate. We compared decidualized cellular morphology between the Ncoa6 knockdown cells and control cells. Messenger RNA (mRNA) sequencing was performed to determine the Ncoa6 target genes in undecidualized HESCs. We found that the knockdown of Ncoa6 caused the failure of morphological changes in decidualized HESCs compared to that in the control cells. mRNA sequencing revealed that Ncoa6 regulates the expression of genes associated with the regulation of actin fibers. Ncoa6 knockdown cells failed to reorganize actin fibers during the decidualization of HESCs. Ncoa6 was shown to play an essential role in decidualization via the appropriate regulation of actin fiber regulation in HESCs. Herein, our in vitro studies revealed a part of the mechanisms involved in endometrial decidualization. Future research is needed to investigate these mechanisms in women with implantation defects.
影响因子：
4.2
作者：
Diana Anderson
通讯作者：
Diana Anderson