Group Testing for Severe Acute Respiratory Syndrome-Coronavirus 2 to Enable Rapid Scale-up of Testing and Real-Time Surveillance of Incidence
Group Testing for Severe Acute Respiratory Syndrome-Coronavirus 2 to Enable Rapid Scale-up of Testing and Real-Time Surveillance of Incidence
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DOI：
10.1093/infdis/jiaa378
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发表时间：
2020-09-15
影响因子：
6.4
通讯作者：
Hudgens, Michael G.
中科院分区：
文献类型：
--
作者：
Pilcher, Christopher D.;Westreich, Daniel;Hudgens, Michael G.
可通过群体检测实现对严重急性呼吸综合征-冠状病毒2型（SARS-CoV-2）的高通量分子检测，在群体检测中筛选样本池，只有在样本池检测呈阳性后才对单个样本进行检测。一些实验室最近发表了将汇集策略应用于SARS-CoV-2标本的例子，但缺乏关于有效汇集策略的总体指导。因此，我们基于现有的sars - cov -2病毒动力学数据建立了样本池算法的效率和准确性模型。对于固定数量的测试，我们估计使用群体测试的程序可以筛选2-20倍于个体测试的样本，增加确定的真阳性感染总数，并提高结果的阳性预测值。我们比较了在不同测试情况下可能预期的结果，并提供了小组测试实施的一般建议。提供了一个免费的、公开的网络计算器，以帮助告知实验室对SARS-CoV-2池算法的决策。
High-throughput molecular testing for severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) may be enabled by group testing in which pools of specimens are screened, and individual specimens tested only after a pool tests positive. Several laboratories have recently published examples of pooling strategies applied to SARS-CoV-2 specimens, but overall guidance on efficient pooling strategies is lacking. Therefore we developed a model of the efficiency and accuracy of specimen pooling algorithms based on available data on SAR-CoV-2 viral dynamics. For a fixed number of tests, we estimate that programs using group testing could screen 2-20 times as many specimens compared with individual testing, increase the total number of true positive infections identified, and improve the positive predictive value of results. We compare outcomes that may be expected in different testing situations and provide general recommendations for group testing implementation. A free, publicly-available Web calculator is provided to help inform laboratory decisions on SARS-CoV-2 pooling algorithms.