Directed movement toward, translocation along, penetration into and exit from vascular networks by breast cancer cells in 3D.
Directed movement toward, translocation along, penetration into and exit from vascular networks by breast cancer cells in 3D.
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DOI：
10.1080/19336918.2021.1957527
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发表时间：
2021-12
影响因子：
3.2
通讯作者：
Soll DR
中科院分区：
文献类型：
--
作者：
Wessels DJ;Pujol C;Pradhan N;Lusche DF;Gonzalez L;Kelly SE;Martin EM;Voss ER;Park YN;Dailey M;Sugg SL;Phadke S;Bashir A;Soll DR
我们开发了一个计算机辅助平台，使用激光扫描共聚焦显微镜实时三维重建转移性乳腺癌细胞与人脐静脉内皮细胞(HUVECs)之间的相互作用。我们证明，MB-231癌细胞在丝状伪足的促进下向HUVEC网络迁移，沿着网络表面迁移，穿透并在HUVEC网络内迁移，退出并继续沿着网络表面迁移。该系统非常适合3D重建和计算分析，以及对潜在的抗转移单抗和其他药物的效果进行评估。我们证明了抗rhamm抗体可以阻止丝状基座的形成，我们发现的所有行为都发生在MB-231细胞和HUVEC网络之间。
We developed a computer-assisted platform using laser scanning confocal microscopy to 3D reconstruct in real-time interactions between metastatic breast cancer cells and human umbilical vein endothelial cells (HUVECs). We demonstrate that MB-231 cancer cells migrate toward HUVEC networks, facilitated by filopodia, migrate along the network surfaces, penetrate into and migrate within the HUVEC networks, exit and continue migrating along network surfaces. The system is highly amenable to 3D reconstruction and computational analyses, and assessments of the effects of potential anti-metastasis monoclonal antibodies and other drugs. We demonstrate that an anti-RHAMM antibody blocks filopodium formation and all of the behaviors that we found take place between MB-231 cells and HUVEC networks.
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影响因子：
3.2
作者：
Arjonen, Antti;Kaukonen, Riina;Ivaska, Johanna
通讯作者：
Ivaska, Johanna
影响因子：
4.8
作者：
Chen, Chia-Chi;Chen, Li-Li;Huang, Tze-Sing
通讯作者：
Huang, Tze-Sing
影响因子：
15.9
作者：
BROOKS, PC;STROMBLAD, S;CHERESH, DA
通讯作者：
CHERESH, DA
影响因子：
3.7
作者：
Cojoc D;Difato F;Ferrari E;Shahapure RB;Laishram J;Righi M;Di Fabrizio EM;Torre V
通讯作者：
Torre V
影响因子：
8.8
作者：
Arwert EN;Harney AS;Entenberg D;Wang Y;Sahai E;Pollard JW;Condeelis JS
通讯作者：
Condeelis JS