QUANTITATIVE-ANALYSIS OF THE VARIABILITY OF SUBSTANTIA-NIGRA PIGMENTED CELL CLUSTERS IN THE HUMAN
QUANTITATIVE-ANALYSIS OF THE VARIABILITY OF SUBSTANTIA-NIGRA PIGMENTED CELL CLUSTERS IN THE HUMAN
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DOI：
10.1016/0306-4522(95)00163-d
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发表时间：
1995-09-01
期刊：
影响因子：
3.3
通讯作者：
CARTWRIGHT, H
中科院分区：
文献类型：
--
作者：
MCRITCHIE, DA;HALLIDAY, GM;CARTWRIGHT, H
尽管人类黑质在神经退行性疾病中发挥着关键作用，但目前对于其内部组织尚未达成共识。我们使用连续切片分析和计算机重建定量分析了人类黑质中黑色素色素神经元簇模式的变异性。黑质致密部显示出由内侧部和外侧部以及背侧和腹侧层组成的双层组织，如前所述[D. A.麦克里奇等人。 (1995) J.comp。内罗尔。 （正在印刷中）]。根据位置和细胞密度，背侧和腹侧层都可以进一步细分为三个内侧细胞柱。这些细胞簇的存在和排列在横切面（目前用于诊断神经病理学的平面）中变化最大。因此，对人类黑质单个横切面内细胞损失的地形模式进行定量评估应谨慎对待。相反，在水平切片中可以看到细胞柱的完整轴尾范围。因此，尽管在大多数切片中只发现了两个细胞柱，但在该剖面中发现了每个区域大量色素神经元的一致样本。我们的结果表明，利用黑质的水平切片可以实现更高的定量可靠性。
At present there is no consensus concerning the internal organization of the human substantia nigra, despite its pivotal role in neurodegenerative conditions. We have quantitatively analysed the variability in the pattern of clusters of melanin-pigmented neurons in the human substantia nigra using serial section analysis and computer reconstructions. The substantia nigra pars compacta showed a bilaminar organization consisting of the pars medialis and pars lateralis, as well as dorsal and ventral tiers as described previously [D. A. McRitchie et al. (1995) J. comp. Neurol. (in press)]. Both the dorsal and ventral tiers could be further subdivided into three mediolateral cell columns based on position and cell density. The presence and arrangement of these cell clusters was most variable in transverse sections (the plane currently used for diagnostic neuropathology).Quantitative assessment of the topographical pattern of cell loss within single transverse sections of the human substantia nigra should therefore be treated with some caution. In contrast, the full rostrocaudal extent of the cell columns could be seen in horizontal sections. Thus, consistent samples of larger numbers of pigmented neurons per region were found in this section plane, although only two cell columns were found in most sections. Our results show that greater quantitative reliability can be achieved with horizontal sections of the substantia nigra.