A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
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DOI：
10.3791/59295
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发表时间：
2019-04-01
影响因子：
1.2
通讯作者：
Pollock, John A.
中科院分区：
文献类型：
--
作者：
Saleem, Muzamil;Stevens, Andrea M.;Pollock, John A.
将药剂静脉（IV）给药至大鼠尾静脉可能既困难又不一致。优化尾静脉注射是许多需要将试剂直接引入血流的实验程序的关键部分。在不知情的情况下，注射可以是皮下的，这可能会改变科学结果。利用纳米乳液为基础的生物探针与纳入近红外荧光（NIRF）染料，这种方法提供了成像成功的尾静脉注射在体内的能力。通过使用NIRF成像仪，在药剂注射之前和之后拍摄图像。然后根据注射部位NIRF信号的强度定性或定量确定可接受的IV注射。
Intravenous (IV) administration of agents into the tail vein of rats can be both difficult and inconsistent. Optimizing tail vein injections is a key part of many experimental procedures where reagents need to be introduced directly into the bloodstream. Unwittingly, the injection can be subcutaneous, possibly altering the scientific outcomes. Utilizing a nanoemulsion-based biological probe with an incorporated near-infrared fluorescent (NIRF) dye, this method offers the capability of imaging a successful tail vein injection in vivo. With the use of a NIRF imager, images are taken before and after the injection of the agent. An acceptable IV injection is then qualitatively or quantitatively determined based on the intensity of the NIRF signal at the site of injection.