Virus-Induced Alterations in Primary Metabolism Modulate Susceptibility to Tobacco rattle virus in Arabidopsis
Virus-Induced Alterations in Primary Metabolism Modulate Susceptibility to Tobacco rattle virus in Arabidopsis
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DOI：
10.1104/pp.114.250340
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发表时间：
2014-12-01
期刊：
影响因子：
7.4
通讯作者：
Llave, Cesar
中科院分区：
文献类型：
--
作者：
Fernandez-Calvino, Lourdes;Osorio, Sonia;Llave, Cesar
在相容病毒感染期间，植物通过重编程基因表达和代谢物含量做出反应。虽然对基因表达进行了大量研究，但我们对病毒存在时发生的代谢变化的了解有限。本研究将烟草摇铃病毒（TRV）侵染的拟南芥（Arabidopsis thaliana）的基因表达与代谢物分析相结合，探讨初级代谢对病毒侵染的影响。我们的研究结果表明，在TRV感染期间，初级代谢在许多方面被重新配置，这反映在糖和氨基酸水平的显著变化上。多变量数据分析显示，这些变化在TRV最大积累的时间点尤为明显，尽管感染时间是变异的主要来源。此外，TRV还引起了叶片脂质和脂肪酸组成的变化。我们发现，一些缺乏支链氨基酸分解代谢或脂肪酸代谢的拟南芥突变体对TRV的易感性发生了改变。最后，我们发现trv感染植株中腐胺含量的增加与trv感染植株对冰冻胁迫的耐受性增强有关，并且腐胺生物合成的损害促进了病毒的增殖。因此，我们的结果为更好地理解初级代谢与病毒感染之间的关系提供了一个有趣的概述。
During compatible virus infections, plants respond by reprogramming gene expression and metabolite content. While gene expression studies are profuse, our knowledge of the metabolic changes that occur in the presence of the virus is limited. Here, we combine gene expression and metabolite profiling in Arabidopsis (Arabidopsis thaliana) infected with Tobacco rattle virus (TRV) in order to investigate the influence of primary metabolism on virus infection. Our results revealed that primary metabolism is reconfigured in many ways during TRV infection, as reflected by significant changes in the levels of sugars and amino acids. Multivariate data analysis revealed that these alterations were particularly conspicuous at the time points of maximal accumulation of TRV, although infection time was the dominant source of variance during the process. Furthermore, TRV caused changes in lipid and fatty acid composition in infected leaves. We found that several Arabidopsis mutants deficient in branched-chain amino acid catabolism or fatty acid metabolism possessed altered susceptibility to TRV. Finally, we showed that increments in the putrescine content in TRV-infected plants correlated with enhanced tolerance to freezing stress in TRV-infected plants and that impairment of putrescine biosynthesis promoted virus multiplication. Our results thus provide an interesting overview for a better understanding of the relationship between primary metabolism and virus infection.