The role of Celsr3 in the development of central somatosensory projections from dorsal root ganglia
The role of Celsr3 in the development of central somatosensory projections from dorsal root ganglia
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Celsr3 在背根神经节中枢体感投射发育中的作用
DOI：
10.1016/j.neuroscience.2017.07.040
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发表时间：
2017-09
期刊：
影响因子：
3.3
通讯作者：
Zhou L
中科院分区：
文献类型：
--
作者：
Wang F;Wang Q;Li C;Yu P;Qu Y;Zhou L
背根神经节（DRG）神经元接收外周体感信息并将有序投射发送到脊髓和脑干中的二级中继核。已知非典型钙粘蛋白 Celsr3 在几个中央和外周轴突的布线中发挥关键作用。尽管 Celsr3mRNA 在 DRG 神经元中大量表达，但其在体感投射发育中的作用仍未被探索。在这里，我们使用 Wnt1-Cremice 杂交中的条件基因失活来评估 Celsr3 在 DRG 中的作用。使用 Celsr3-GFP 转基因小鼠，我们发现 Celsr3 在不同的 DRG 细胞中高表达，例如 Pavalbumin、TrkB 和降钙素基因相关肽 (CGRP) 阳性神经元。Wnt1-Cre;Celsr3f/− 动物存活了数周，并且看起来比同窝对照小。 DiI 追踪显示，在突变小鼠和对照小鼠中，早期 DRG 轴突进入脊髓并到达脊髓目标。与对照相比，出生后第 7 天和第 14 天，突变体脊髓 I 层中 CGRP 阳性纤维密度显着降低。此外，与对照样本相比，突变体中更多的新蛋白阳性纤维侵入灰质，并与脊髓运动神经元有更多的接触。行为分析表明，与对照组相比，突变动物对疼痛不太敏感，对机械刺激更敏感。总之，Celsr3 对于中央 DRG 投影的图案化是可有可无的，但它调节灰质中感觉纤维的精细映射，这对于体感处理很重要。
Dorsal root ganglion (DRG) neurons receive peripheral somatosensory information and send orderly projections to second-order relay nuclei in the spinal cord and in the brainstem. Atypical cadherinCelsr3is known to play a critical role in wiring of several central and peripheral axons. AlthoughCelsr3mRNA is heavily expressed in DRG neurons, its role in the development of somatosensory projections remains unexplored. Here we assessed the role ofCelsr3in DRG using conditional gene inactivation in crosses withWnt1-Cremice. UsingCelsr3-GFPtransgenic mice, we found that Celsr3 was highly expressed in different DRG cells, such as Pavalbumin-, TrkB-, and calcitonin gene-related peptide (CGRP)-positive neurons.Wnt1-Cre;Celsr3f/−animals survived for a few weeks and looked smaller than littermate controls. DiI tracing showed that early DRG axons entered the spinal cord and reached spinal cord targets similarly in mutant and control mice. CGRP-positive fiber density was significantly decreased in lamina I in the mutant versus control spinal cord at postnatal day (P) 7 and P14. Furthermore, more Pavalbumin-positive fibers invaded the gray matter and made more contacts with spinal motor neurons in mutant than in control samples. Behavioral analysis showed that mutant animals were less sensitive to pain and more sensitive to mechanical stimulation than controls. In conclusion, Celsr3 is dispensable for the patterning of central DRG projections, but it regulates for the fine mapping of sensory fibers in the gray matter, which is important for somatosensory processing.
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影响因子：
5.3
作者：
Onishi, Keisuke;Shafer, Beth;Zou, Yimin
通讯作者：
Zou, Yimin
DOI：
10.1523/jneurosci.4508-10.2010
发表时间：
2010-11-24
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Fenstermaker AG;Prasad AA;Bechara A;Adolfs Y;Tissir F;Goffinet A;Zou Y;Pasterkamp RJ
通讯作者：
Pasterkamp RJ
DOI：
10.1093/cercor/bhw132
发表时间：
2016-07
期刊：
Cerebral cortex (New York, N.Y. : 1991)
影响因子：
--
作者：
Feng J;Xian Q;Guan T;Hu J;Wang M;Huang Y;So KF;Evans SM;Chai G;Goffinet AM;Qu Y;Zhou L
通讯作者：
Zhou L
影响因子：
4.6
作者：
Hancock, Melissa L.;Nowakowski, Dan W.;Flanagan, John G.
通讯作者：
Flanagan, John G.
影响因子：
5.3
作者：
G. Ying;Sen Wu;Ruiqing Hou;Wei Huang;M. Capecchi;Qiang Wu
通讯作者：
G. Ying;Sen Wu;Ruiqing Hou;Wei Huang;M. Capecchi;Qiang Wu