Juvenile hormone-dependent Kazal-type serine protease inhibitor Greglin safeguards insect vitellogenesis and egg production
Juvenile hormone-dependent Kazal-type serine protease inhibitor Greglin safeguards insect vitellogenesis and egg production
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保幼激素依赖性 Kazal 型丝氨酸蛋白酶抑制剂 Greglin 保障昆虫卵黄发生和产蛋
DOI：
10.1096/fj.201801068r
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发表时间：
2019-01-01
期刊：
影响因子：
4.8
通讯作者：
Zhou, Shutang
中科院分区：
文献类型：
--
作者：
Guo, Wei;Wu, Zhongxia;Zhou, Shutang
除了防止昆虫变态外，保幼激素 (JH) 还可以刺激昆虫繁殖。然而，JH 在昆虫繁殖中作用的分子机制仍然很大程度上未知。通过重新分析飞蝗成虫和其他发育阶段的转录组数据，我们鉴定了编码 Kazal 型蛋白酶抑制剂的基因，该基因以前称为 Greglin。 Greglin 在成年女性中特异性表达，在脂肪体和卵巢中含量最多。有趣的是，Greglin 是成年雌性蝗虫中高表达基因的前 3 位，仅次于 2 个卵黄蛋白原 (Vg) 基因。 Greglin 受 JH 诱导，并在卵黄发生阶段以非常高的水平表达。成年雌性蝗虫中 Greglin 的敲低会导致丝氨酸蛋白酶底物加速降解，并显着降低血淋巴和卵巢中 Greglin 蛋白的水平。随之而来的表型包括卵母细胞成熟受阻、卵巢生长停滞和卵泡上皮萎缩，以及卵子数量和孵化率下降。据我们所知，这些数据提供了第一个证据，表明 JH 依赖性 Greglin 可能通过保护卵黄发生和其他形式的卵黄前体免受蛋白水解而参与蝗虫卵黄发生和卵母细胞成熟。该结果为JH的调节和蛋白酶抑制剂在昆虫卵黄发生、卵母细胞成熟和繁殖中的功能提供了新的见解。-Guo，W.，Wu，Z.，Yang，L.，Cai，Z.，Zhao，L.，Zhou，S.保幼激素依赖性Kazal型丝氨酸蛋白酶抑制剂Greglin保障昆虫卵黄发生和卵子 生产。 FASEB J. 33, 917–927 (2019)。 www.fasebj.org
In addition to preventing insect metamorphosis, juvenile hormone (JH) is known to stimulate aspects of insect reproduction. However, the molecular mechanisms of JH action in insect reproduction remain largely unknown. By reanalyzing the transcriptomic data from adults and other developmental stages of the migratory locust Locusta migratoria, we identified a gene coding for Kazal‐type protease inhibitor, previously named Greglin. Greglin is specifically expressed in adult females and most abundant in the fat body and ovaries. Interestingly, Greglin is among the top 3 of highly expressed genes in adult female locusts, after 2 vitellogenin (Vg) genes. Greglin is induced by JH and expressed at remarkably high levels in the vitellogenic stage. Knockdown of Greglin in adult female locusts results in accelerated degradation of serine protease substrate and significantly reduced levels of Greglin protein in hemolymph and ovaries. The consequent phenotypes include blocked oocyte maturation, arrested ovarian growth and shrunken follicular epithelium, as well as declines in egg number and hatchability. The data provide the first evidence, to our knowledge, that JH‐dependent Greglin is involved in locust vitellogenesis and oocyte maturation likely by protecting vitellogenesis and other forms of yolk precursors from proteolysis. The result offers new insights into the regulation of JH and function of protease inhibitors in insect vitellogenesis, oocyte maturation and fecundity.—Guo, W., Wu, Z., Yang, L., Cai, Z., Zhao, L., Zhou, S. Juvenile hormone–dependent Kazal‐type serine protease inhibitor Greglin safeguards insect vitellogenesis and egg production. FASEB J. 33, 917–927 (2019). www.fasebj.org