Aerosolized Pseudomonas elastase and lung fluid balance in anesthetized sheep.
Aerosolized Pseudomonas elastase and lung fluid balance in anesthetized sheep.
复制标题
麻醉羊雾化假单胞菌弹性蛋白酶和肺液平衡。
DOI：
10.1152/jappl.1992.72.5.1927
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Azghani,AO
中科院分区：
文献类型：
--
作者：
Peterson,BT;Collins,ML;Gray,LD;Azghani,AO
通过用20 mg来自铜绿假单胞菌的弹性蛋白酶（Ps.弹性蛋白酶）以增加肺上皮通透性而不影响肺内皮通透性或肺血管压力。PS.弹性蛋白酶对肺血管压力、肺泡-动脉PO 2梯度（A-aPO 2）、肺淋巴液流量或蛋白质浓度、或死后肺的水量没有影响。这些绵羊的形态肺泡淹没评分是对照水平的2.5倍，但这只是轻微的显着性。仅用20 cmH 2 O升高左心房压可增加死后肺水量，但对A-aPO 2、肺泡泛红评分或肺上皮通透性（通过99 mTc标记人血清白蛋白清除率评估）无影响。添加雾化Ps。弹性蛋白酶对这些绵羊的总肺水量没有影响，但它引起了水重新分配到空气空间，这可以通过肺泡淹没评分和A-aPO 2的显著增加来证明（P <0.01）。在无弹性蛋白酶的情况下，左心房压力升高40 cmH 2 O引起的反应与在有弹性蛋白酶的情况下左心房压力升高20 cmH 2 O引起的反应相同，除了更高的压力引起肺总水量的更大增加。我们的结论是改变肺上皮的完整性与雾化Ps。弹性蛋白酶引起肺水重新分布到肺泡中而不影响总肺水量。（250字处删节）
The role of the lung epithelium in lung fluid balance was studied by ventilating anesthetized sheep with an aerosol of 20 mg of elastase from Pseudomonas aeruginosa (Ps. elastase) to increase lung epithelial permeability without affecting lung endothelial permeability or lung vascular pressures. Ps. elastase had no effect on the lung vascular pressures, the alveolar-arterial PO2 gradient (A-aPO2), the flow or protein concentration of the lung lymph, or the postmortem water volume of the lungs. The morphological alveolar flooding score in these sheep was 2.5 times the control level, but this was only marginally significant. Elevation of the left atrial pressure by 20 cmH2O alone increased the postmortem lung water volume but had no effect on A-aPO2, the alveolar flooding score, or the lung epithelial permeability assessed by the clearance of 99mTc-labeled human serum albumin. Addition of aerosolized Ps. elastase to these sheep had no effect on the total lung water volume, but it caused a redistribution of water into the air spaces, as evidenced by significant increases in the alveolar flooding score and A-aPO2 (P less than 0.01). Elevation of the left atrial pressure by 40 cmH2O without elastase caused the same response as elevation of the left atrial pressure by 20 cmH2O with elastase, except the higher pressure caused a greater increase in the total lung water volume. We conclude that alteration of the integrity of the lung epithelium with aerosolized Ps. elastase causes a redistribution of lung water into the alveoli without affecting the total lung water volume.(ABSTRACT TRUNCATED AT 250 WORDS)