Construction of a Vertebrate Embryo from Two Opposing Morphogen Gradients
Construction of a Vertebrate Embryo from Two Opposing Morphogen Gradients
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DOI：
10.1126/science.1248252
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发表时间：
2014-04-04
期刊：
影响因子：
56.9
通讯作者：
Thisse, Christine
中科院分区：
文献类型：
--
作者：
Xu, Peng-Fei;Houssin, Nathalie;Thisse, Christine
脊椎动物胚胎的发育涉及严格调控的分子和细胞过程，这些过程逐步指导增殖的胚胎细胞关于它们的身份和行为。尽管已经发现许多基因活性在早期胚胎发生过程中是必不可少的，但对于足以指导多能细胞组织胚胎的最低条件和因素知之甚少。在这里，我们表明，骨形态发生蛋白（BMP）和Nodal，两个转化生长因子家族成员，作为形态发生剂的相对梯度，足以诱导组织所需的分子和细胞机制，在体内或体外，未提交的细胞的斑马鱼囊胚动物极成一个发育良好的胚胎。
Development of vertebrate embryos involves tightly regulated molecular and cellular processes that progressively instruct proliferating embryonic cells about their identity and behavior. Whereas numerous gene activities have been found to be essential during early embryogenesis, little is known about the minimal conditions and factors that would be sufficient to instruct pluripotent cells to organize the embryo. Here, we show that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo.