High-contrast Noninvasive Imaging of Kidney Clearance Kinetics Enabled by Renal Clearable Nanofluorophores.
High-contrast Noninvasive Imaging of Kidney Clearance Kinetics Enabled by Renal Clearable Nanofluorophores.
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DOI：
10.1002/anie.201507868
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发表时间：
2015-12-14
期刊：
影响因子：
--
通讯作者：
Zheng J
中科院分区：
文献类型：
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作者：
Yu M;Liu J;Ning X;Zheng J
肾透明探头肾清除动力学(KCK)的无创性成像是研究单侧肾功能疾病的关键，但在活体荧光成像方面具有很高的挑战性。虽然这一长期存在的挑战通常被归因于有限的光穿透深度，但我们发现，传统染料在皮肤中的快速和持续积累“阴影”了来自肾脏的真实荧光信号，并阻止了KCK的非侵入性成像，然而，这可以通过肾脏可清除的纳米荧光团来解决。通过将近红外发射与有效的肾脏清除和超低本底干扰相结合，纳米荧光团可以将肾脏对比增强和成像时间窗分别提高约50倍和~1000倍，并显著减少无创和有创KCK之间的偏差，为体内荧光成像在临床前无创肾功能评估中的转化奠定了基础。
Noninvasive imaging of kidney clearance kinetics (KCK) of renal clearable probes is key to studying unilateral kidney functional diseases but is highly challenging to be achieved with in vivo fluorescence imaging. While this long-standing challenge is often attributed to the limited light penetration depth, we found that rapid and persistent accumulation of conventional dyes in the skin “shadowed” real fluorescence signals from the kidneys and prevented noninvasive imaging of KCK, which, however, can be addressed with renal clearable nanofluorophores. By integrating near infrared emission with efficient renal clearance and ultralow background interference, the nanofluorophores can increase kidney-contrast enhancement and imaging-time window by ~50 and ~1000 times over conventional dyes and significantly minimize deviation between noninvasive and invasive KCK, laying down a foundation for translating in vivo fluorescence imaging in preclinical noninvasive kidney function assessment.