Enantioanalysis of glyceric acid using enantioselective, potentiometric membrane electrodes : applications for the diagnosis of PH II and D-glyceric acidemia/acidurias
Enantioanalysis of glyceric acid using enantioselective, potentiometric membrane electrodes : applications for the diagnosis of PH II and D-glyceric acidemia/acidurias
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DOI：
10.1016/j.snb.2004.09.025
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发表时间：
2005-05
影响因子：
8.4
通讯作者：
R. Stefan;R. M. Nejem
中科院分区：
文献类型：
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作者：
R. Stefan;R. M. Nejem
D -甘油和l-甘油是两种不同疾病的人体代谢物。过量的d-GA排泄引起d-甘油中毒/酸尿症，而过量的l-GA排泄引起2型高草酸尿症，PH II。提出了基于麦芽糖糊精I（葡萄糖当量（DE） 4.0-7.0）、II （DE 13.0-17.0）和III （DE 16.5-19.5）作为手性选择体的对映选择性电位膜电极（EPMEs），用于测定l-甘油（基于麦芽糖糊精I和III的EPMEs）和d-甘油（基于麦芽糖糊精II的EPME）酸（l-和d-GA）。基于麦芽糖糊精I和III的EPMEs可以可靠地用于直接电位法分析L - ga，浓度范围分别为10−8 ~ 10−6和10−6 ~ 10−3mol/L，检出限极低（分别为1.19×10−9和1.0×10−7mol/L）。基于麦芽糖糊精II的EPME在10−5 ~ 10−3mol/L浓度范围内对d-GA进行了对映分析，检测限为1×10−6mol/L。测定了EPMEs对l-甘油酸（或d-甘油酸）的对映选择性，对肌酸、肌酐和Na+、K+、Ca2+等无机阳离子的选择性。简单地抛光以获得准备用于新测量的新鲜表面可以再生电极表面。
d- and l-glyceric acids are human metabolites responsible for two different diseases. Excess excretion of d-GA causes d-glyceric academia/acidurias, while excess excretion of l-GA causes hyperoxaluria type 2, PH II. Enantioselective, potentiometric membrane electrodes (EPMEs) based on maltodextrins I (dextrose equivalence (DE) 4.0–7.0), II (DE 13.0–17.0) and III (DE 16.5–19.5) as chiral selectors are proposed for the determination of l-glyceric (EPMEs based on maltodextrins I and III) and d-glyceric (EPME based on maltodextrin II) acid (l- and d-GA). EPMEs based on maltodextrins I and III can be reliably used for the analyses of l-GA using direct potentiometric method, in the concentration range of 10−8to 10−6and 10−6to 10−3mol/L, respectively, with very low detection limits (1.19×10−9and 1.0×10−7mol/L, respectively). The EPME based on maltodextrin II was successfully used for the enantioanalysis of d-GA in the 10−5to 10−3mol/L concentration range with detection limit of 1×10−6mol/L. The enantioselectivity of EPMEs was determined over l-glyceric acid (or d-glyceric acid), and their selectivity over creatine, creatinine and some inorganic cations such as Na+, K+and Ca2+. Simply polishing to obtain a fresh surface ready to be used in a new measurement can regenerate the surface of the electrodes.