Two enhancer domains control early aspects of the complex expression pattern of Msx1
Two enhancer domains control early aspects of the complex expression pattern of Msx1
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DOI：
10.1016/s0925-4773(96)00646-6
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发表时间：
1997-02-01
影响因子：
2.6
通讯作者：
Hill, RE
中科院分区：
文献类型：
--
作者：
MacKenzie, A;Purdie, L;Hill, RE
为了了解Msx1基因复杂的胚胎表达模式是如何产生的，我们对Msx1基因周围13kb的DNA进行了转基因分析。大多数Msx1基因的广泛表达模式是通过将LacZ基因与5kb的Msx1 5‘侧翼DNA融合在一起在转基因中复制的。确定了产生这种模式的两个增强子结构域。远端元件在第一弓和鼻黏膜上皮细胞中表达，并被限制在240bp的范围内。而在鼻浅上皮、背侧和腹侧肌节、四肢间充质、眼、耳、顶板、第二弓、生殖器脊和骨痂表达的近端元件只有78bp。(C)1997年爱思唯尔爱尔兰科学有限公司。
To understand how the complex embryonic expression pattern of the Msx1 gene is produced a transgenic analysis of 13 kb of DNA around the Msx1 locus was carried out. Most of the extensive expression pattern of the Msx1 gene was reproduced in transgenics using the LacZ gene fused to 5 kb of Msx1 5' flanking DNA. Two enhancer domains were identified which produced this pattern. The distal element produced expression in the first arch and the nasal epithelium and was restricted to 240 bp. However, the proximal element which produced expression in superficial nasal epithelium, dorsal and ventral myotome, limb mesenchyme, eye, ear, roof plate, second arch, genital ridge and epiphysis, was contained in only 78 bp. (C) 1997 Elsevier Science Ireland Ltd.