Comprehensive Analysis and Expression Profiling of the OsLAX and OsABCB Auxin Transporter Gene Families in Rice (Oryza sativa) under Phytohormone Stimuli and Abiotic Stresses.
Comprehensive Analysis and Expression Profiling of the OsLAX and OsABCB Auxin Transporter Gene Families in Rice (Oryza sativa) under Phytohormone Stimuli and Abiotic Stresses.
复制标题
DOI：
10.3389/fpls.2016.00593
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Subudhi PK
中科院分区：
文献类型：
--
作者：
Chai C;Subudhi PK
植物激素生长素调节植物生长和发育过程的许多方面。植物生长素通过OsLAX、OsPIN和OsABCB三种生长素转运蛋白进行极性运输，形成生长素梯度。我们在这里报告了使用生物信息学工具、公开可获得的微阵列数据和定量RT-PCR对两个水稻生长素转运蛋白基因家族OsLAX和OsABCB的分析。水稻基因组中有5个OsLAXs和22个OsABCBs，分布在8条染色体上。OsLAX基因的外显子-内含子结构和推导蛋白的性质在禾本科植物中相对保守，而OsABCB基因的外显子-内含子结构和推导蛋白的性质在禾本科植物中差异较大。在OsLAXs和OsABCBs中观察到组成型和器官/组织特异性表达模式。对进化上密切相关的“基因对”以及器官/组织特异性表达的分析揭示了水稻进化过程中可能的“功能获得”和“功能丧失”事件。大多数OsLAX和OsABCB基因受到干旱和盐胁迫以及激素刺激[生长素和脱落酸（阿坝）]的调控，这表明非生物胁迫和激素信号通路之间存在广泛的串扰。这些基因启动子区存在大量的生长素和胁迫相关的顺式调控元件，这可能是它们对环境刺激产生大量响应的原因，表明这些基因在发育和生理过程中调控网络的复杂性。本研究对OsLAX和OsABCB生长素转运蛋白基因的综合分析，将有助于理解这些基因家族在激素信号传导和水稻适应逆境中的生物学意义。
The plant hormone auxin regulates many aspects of plant growth and developmental processes. Auxin gradient is formed in plant as a result of polar auxin transportation by three types of auxin transporters such as OsLAX, OsPIN, and OsABCB. We report here the analysis of two rice auxin transporter gene families, OsLAX and OsABCB, using bioinformatics tools, publicly accessible microarray data, and quantitative RT-PCR. There are 5 putative OsLAXs and 22 putative OsABCBs in rice genome, which were mapped on 8 chromosomes. The exon-intron structure of OsLAX genes and properties of deduced proteins were relatively conserved within grass family, while that of OsABCB genes varied greatly. Both constitutive and organ/tissue specific expression patterns were observed in OsLAXs and OsABCBs. Analysis of evolutionarily closely related “gene pairs” together with organ/tissue specific expression revealed possible “function gaining” and “function losing” events during rice evolution. Most OsLAX and OsABCB genes were regulated by drought and salt stress, as well as hormonal stimuli [auxin and Abscisic Acid (ABA)], which suggests extensive crosstalk between abiotic stresses and hormone signaling pathways. The existence of large number of auxin and stress related cis-regulatory elements in promoter regions might account for their massive responsiveness of these genes to these environmental stimuli, indicating complexity of regulatory networks involved in various developmental and physiological processes. The comprehensive analysis of OsLAX and OsABCB auxin transporter genes in this study would be helpful for understanding the biological significance of these gene families in hormone signaling and adaptation of rice plants to unfavorable environments.