Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3
Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3
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DOI：
10.1016/j.carbpol.2012.05.063
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发表时间：
2012-09-01
影响因子：
11.2
通讯作者：
Ahmed, Nuzhat
中科院分区：
文献类型：
--
作者：
Ahmed, Rifat Z.;Siddiqui, Khaizran;Ahmed, Nuzhat
在含有蔗糖的DeMan、Rogosa和Sharpe（MRS）琼脂上从甘蓝中分离到产胞外多糖（EPS）的Weissella cibaria CMGDEX 3。葡聚糖蔗糖酶活性和葡聚糖产量分别为7.1 DSU ml（-1）和2.4 g dl（-1）。通过FTIR对纯化后的EPS进行结构表征。H-1和C-13 NMR谱表明W. cibaria CMGDEX 3合成了主要具有α（1 -> 6）糖苷键且仅具有少量（3.4%）α（1 -> 3）连接分支的线性葡聚糖。分子量测定表明，它是一种高分子量的右旋糖酐，平均分子量大于200万Da，据我们所知，这是首次从巴基斯坦分离到合成右旋糖酐的Weissella属。(C)2012爱思唯尔有限公司保留所有权利。
Exopolysaccharide (EPS) producing Weissella cibaria CMGDEX3 was isolated from cabbage on sucrose containing De Man, Rogosa and Sharpe (MRS) agar. Dextransucrase activity and dextran yield was found to be 7.1 DSU ml(-1) and 2.4 g dl(-1), respectively. The structural characterization of purified EPS determined by FTIR. H-1 and C-13 NMR spectroscopy demonstrated that W. cibaria CMGDEX3 synthesized a linear dextran that predominately had alpha (1 -> 6) glycosidic linkages with only a few (3.4%) alpha (1 -> 3) linked branches. Molecular mass determination showed that it was a high molecular weight dextran of an average >2,000,000 Da. According to our knowledge this is the first report on isolation of dextran synthesizing Weissella genus from Pakistan. (C) 2012 Elsevier Ltd. All rights reserved.