HISTONES AND DNA INCREASE SYNCHRONOUSLY IN NEURONS DURING EARLY POSTNATAL-DEVELOPMENT OF THE RAT FOREBRAIN CORTEX
HISTONES AND DNA INCREASE SYNCHRONOUSLY IN NEURONS DURING EARLY POSTNATAL-DEVELOPMENT OF THE RAT FOREBRAIN CORTEX
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DOI：
10.1007/bf00508448
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
KUENZLE, CC
中科院分区：
文献类型：
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作者：
BREGNARD, A;RUCH, F;KUENZLE, CC
细胞光度测量结果表明，组蛋白和DNA的时间坐标积累发生在大鼠皮质神经元之间的最后一个妊娠日和出生后第3周结束。这两种成分在出生前后迅速上升，然后是一个更长时间的合成。这导致成人组蛋白和DNA水平约为3.3个任意单位，而在所有胎儿阶段的神经元前体细胞和来自神经胶质和肝脏的参考二倍体细胞核中发现的任意单位为2个。在出生后第4周的继发性DNA增加，以前怀疑的基础上使用希夫型染色BAO（双-[4-氨基苯基]-1，3，4-恶二唑）的细胞荧光测量，是没有得到证实的本工作。这是因为在相关时期内向侧脑室重复注射[3 H]胸苷后，替代细胞光度DNA测量（UV吸收扫描）和神经元核放射自显影没有证据表明出生后第3周后DNA进一步增加。组蛋白的积累（通过磺胺黄烷细胞荧光测定法测量）也不会持续到第21天之后。DNA和组蛋白合成在第3周结束时停止。组蛋白的电泳分析表明，相对组蛋白组成的变化只有轻微的神经元发育过程中。除了组蛋白H1 °：H1出生前后，组蛋白组成没有发育变化。
Cytophotometric measurements reveal that a temporally coordinate accumulation of histones and DNA occurs in rat cortex neurons between the last gestational day and the end of the 3rd postnatal week. A rapid rise of both constituents around birth is followed by a more protracted synthesis. This leads to adult histone and DNA levels of approximately 3.3 arbitrary units as compared with 2 arbitrary units found in neuronal precursor cells at all fetal stages and in reference diploid nuclei from glia and liver. A secondary DNA increase in the 4th postnatal week, previously suspected on the basis of cytofluorometric measurements using the Schiff-type stain BAO (bis-[4-aminophenyl]-1,3,4-oxadiazole), is not substantiated by the present work. This derives from the finding that alternative cytophotometric DNA measurements (UV absorption scanning) and autoradiographs of neuronal nuclei following repeated injections of [3H]thymidine into the lateral ventricles during the relevant period give no evidence of a further DNA increase after the 3rd postnatal week. Neither does the accumulation of histones (measured by sulfaflavine cytofluorometry) continue beyond day 21. DNA and histone syntheses cease at the end of the 3rd wk. Electrophoretic analyses of the histones show that the relative histone composition changes only slightly during neuronal development. Apart from an increase in the ratio of the histones H1.degree.:H1 around birth no developmental alterations in histone composition are detectable.