SIMULTANEOUS DETERMINATION OF HEMES-A, HEMES-B, AND HEMES-C FROM PYRIDINE HEMOCHROME SPECTRA
SIMULTANEOUS DETERMINATION OF HEMES-A, HEMES-B, AND HEMES-C FROM PYRIDINE HEMOCHROME SPECTRA
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DOI：
10.1016/0003-2697(87)90643-9
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发表时间：
1987-02-15
影响因子：
2.9
通讯作者：
TRUMPOWER, BL
中科院分区：
文献类型：
--
作者：
BERRY, EA;TRUMPOWER, BL
本文介绍了分析吡啶类染料混合物重叠光谱的两种方法，并给出了吡啶类染料的消光系数。在第一个程序中，在与待分析组分数量相等的多个波长处测量吸光度。这是可以计算每种物质浓度的最小光谱数据量。在第二个程序中，在大于待分析组分数量的波长下测量吸光度。这种信息的冗余使得不可能拟合包含来自附加组分的贡献的光谱，除非附加组分的光谱等于被分析物质的光谱的线性组合。这两种方法一般适用于服从比尔定律的组分混合物的绝对光谱或差谱分析。在吸光度测量中对误差的灵敏度仅略大于在单个波长处测量纯组分的灵敏度。
Two procedures for analyzing overlapping optical spectra of mixtures of pyridine hemochromes are described, and extinction coefficients of pyridine hemochromes are provided for use with these methods. In the first procedure, absorbance is measured at a number of wavelengths equal to the number of components to be analyzed. This is the minimum amount of spectral data from which the concentration of each species can be calculated. In the second procedure, absorbance is measured at a number of wavelengths greater than the number of components to be analyzed. This redundancy of information makes it impossible to fit spectra which contain contributions from additional components, unless the spectra of the additional components are equal to linear combinations of the spectra of the species being analyzed. These two procedures are generally applicable to analyses of absolute or difference spectra of mixtures of components obeying Beer''s law. The sensitivity to error in the absorbance measurements is only slightly greater than that for measuring a pure component at a single wavelength.