Covalent Binding of Nanoliposomes to the Surface of Magnetotactic Bacteria for the Synthesis of Self-Propelled Therapeutic Agents
Covalent Binding of Nanoliposomes to the Surface of Magnetotactic Bacteria for the Synthesis of Self-Propelled Therapeutic Agents
复制标题
DOI：
10.1021/nn5011304
复制
发表时间：
2014-05-01
期刊：
影响因子：
17.1
通讯作者：
Tabrizian, Maryam
中科院分区：
文献类型：
--
作者：
Taherkhani, Samira;Mohammadi, Mahmood;Tabrizian, Maryam
靶向和有效地递送治疗剂仍然是控释系统领域未实现的目标。海洋磁球菌MC-1趋磁细菌（MTB）是潜在的治疗载体。通过结合定向趋磁-微嗜气控制这些自我推进的药物，可以超越大分子药物的扩散限制，将更大量的治疗药物输送到实体肿瘤中难以治疗的缺氧区域。这些载体的潜在好处强调需要开发一种适当的方法来附着治疗货物，如载药纳米脂质体，而不会实质性地影响细胞作为递送剂的能力。在这项研究中，我们报告了一种通过碳二亚胺化学将脂质体附着在MTB （MTB- lp）上的策略。研究了MTB-LP的附着效能、运动性和磁响应。结果证实，大量的纳米脂质体（类似于70）与MTB有效地联系在一起，而不影响功能和运动性。使用三种不同细胞类型（J774、NIH/3T3和Colo205）进行的细胞毒性试验显示，与MTB制剂结合的脂质体改善了MTB的生物相容性，而与脂质体的结合并不干扰脂质体的摄取。
The targeted and effective delivery of therapeutic agents remains an unmet goal in the field of controlled release systems. Magnetococcus marinus MC-1 magnetotactic bacteria (MTB) are investigated as potential therapeutic carriers. By combining directional magnetotaxis-microaerophilic control of these self-propelled agents, a larger amount of therapeutics can be delivered surpassing the diffusion limits of large drug molecules toward hard-to-treat hypoxic regions in solid tumors. The potential benefits of these carriers emphasize the need to develop an adequate method to attach therapeutic cargos, such as drug-loaded nanoliposomes, without substantially affecting the cell's ability to act as delivery agents. In this study, we report on a strategy for the attachment of liposomes to MTB (MTB-LP) through carbodiimide chemistry. The attachment efficacy, motility, and magnetic response of the MTB-LP were investigated. Results confirm that a substantial number of nanoliposomes (similar to 70) are efficiently linked with MTB without compromising functionality and motility. Cytotoxicity assays using three different cell types (J774, NIH/3T3, and Colo205) reveal that liposomal attachments to MTB formulation improve the biocompatibility of MTB, whereas attachment does not Interfere with liposomal uptake.