The distinct proteome of placental malaria parasites
The distinct proteome of placental malaria parasites
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DOI：
10.1016/j.molbiopara.2007.05.010
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发表时间：
2007-09-01
影响因子：
1.5
通讯作者：
Duffy, Patrick E.
中科院分区：
文献类型：
--
作者：
Fried, Michal;Hixson, Kim K.;Duffy, Patrick E.
恶性疟原虫感染的红细胞 (IE) 表面表达的疟疾蛋白可介导粘附，并成为保护性免疫反应的目标。在怀孕期间，IE 隔离在胎盘中。胎盘 IE 与硫酸软骨素 A (CSA) 分子结合，并优先转录编码 VAR2CSA 的基因，VAR2CSA 是 PfEMP1 变异表面抗原家族的成员。连续怀孕后，女性会对结合 CSA 的 IE 产生特异性免疫力，并产生针对 VAR2CSA 的抗体。我们使用串联质谱法以及精确质量和时间标签技术来研究胎盘寄生虫的 IE 膜组分。在胎盘 IE 和儿童 IE 中检测到 VAR2CSA pepticles，但 VAR2CSA 的 MC 变体与胎盘 IE 特异性相关。我们鉴定了 6 种保守的假设蛋白，其具有推定的 TM 或信号肽，仅由胎盘 IE 表达，并且有 11 种这样的蛋白在胎盘 IE 中显着更丰富。其中一种假设的蛋白质 PHI 1785w 是一种 42 kDa 的分子，通过蛋白质印迹法在感染孕妇的寄生虫中检测到，但在感染儿童的寄生虫中未检测到。 (C) 2007 Elsevier B.V. 保留所有权利。
Malaria proteins expressed on the surface of Plasmodium falciparum infected erythrocytes (IE) mediate adhesion and are targeted by protective immune responses. During pregnancy, IE sequester in the placenta. Placental IE bind to the molecule chondroitin sulfate A (CSA) and preferentially transcribe the gene that encodes VAR2CSA, a member of the PfEMP1 variant surface antigen family. Over successive pregnancies women develop specific immunity to CSA-binding IE and antibodies to VAR2CSA. We used tandem mass spectrometry together with accurate mass and time tag technology to study IE membrane fractions of placental parasites. VAR2CSA pepticles were detected in placental IE and in IE from children, but the MC variant of VAR2CSA was specifically associated with placental IE. We identified six conserved hypothetical proteins with putative TM or signal peptides that were exclusively expressed by the placental IE, and 11 such proteins that were significantly more abundant in placental IE. One of these hypothetical proteins, PHI 1785w, is a 42 kDa molecule detected by Western blot in parasites infecting pregnant women but not those infecting children. (C) 2007 Elsevier B.V. All rights reserved.