Insecticide resistance increases the vector competence: a case study in Frankliniella occidentalis
Insecticide resistance increases the vector competence: a case study in Frankliniella occidentalis
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杀虫剂抗性提高了媒介能力：西花蓟马的案例研究
DOI：
10.1007/s10340-020-01207-9
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发表时间：
2020-02-20
影响因子：
4.8
通讯作者：
Wu, Qingjun
中科院分区：
文献类型：
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作者：
Wan, Yanran;Zheng, Xiaobin;Wu, Qingjun
西花蓟马是防治番茄斑枯病原点状病毒(TSWV)的最有效媒介。抗药性是害虫管理中普遍存在的问题。然而，昆虫媒介对杀虫剂的抗性与植物病原菌传播之间的相互作用在很大程度上还不清楚。在这项研究中，我们利用一对近等基因系的敏感(IVF03)和抗性(NIL-R)西花链霉菌来评估多杀菌素抗性对TSWV传播的影响。与IVF03相比，NIL-R具有较长的成体前期，但成体寿命较短。更重要的是，与IVF03相比，NIL-R的化蝇率和性别比(女性/男性)显著降低。当给予较短的获取访问期(AAP)6小时时，NIL-R中的病毒繁殖和传播效率显著高于IVF03。相比之下，较长的AAP为96h导致了两种毒株相似的病毒获取和传播效率，尽管TSWV的复制在NIL-R中更大。我们的综合结果表明：(1)大量的适合度成本(例如，降低的化蝇率)与西方斑潜蝇对多杀菌素的抗性有关；然而，(2)杀虫剂抗性提高了媒介能力。
The western flower thrips, Frankliniella occidentalis, is the most efficient vector for Tomato spotted wilt orthotospovirus (TSWV), a devastating plant pathogen. Insecticide resistance is a common issue in pest management. The interactions between insecticide resistance in insect vector and the transmission of plant pathogen, however, are largely unknown. In this study, we assessed the effects of spinosad resistance on TSWV transmission using a pair of near-isogenic lines of susceptible (Ivf03) and resistant (NIL-R) F. occidentalis. NIL-R had a prolonged pre-adult stage, but a shorter adult life span than Ivf03. More importantly, the pupation rate and sex ratio (female/male) in NIL-R were significantly reduced in comparison with Ivf03. When given a shorter acquisition access period (AAP) of 6 h, virus propagation and transmission efficiency in NIL-R were significantly higher than Ivf03. In contrast, a longer AAP of 96 h led to a similar virus acquisition and transmission efficiency for both strains, although TSWV replication was greater in NIL-R. Our combined results suggest that (1) substantial fitness costs (e.g., reduced pupation rate) are associated with spinosad resistance in F. occidentalis ; however, (2) insecticide resistance increases vector competence.