Hapten-specific T-cell responses to 4-hydroxy-3-nitrophenyl acetyl. I. Genetic control of delayed-type hypersensitivity by VH and I-A-region genes
Hapten-specific T-cell responses to 4-hydroxy-3-nitrophenyl acetyl. I. Genetic control of delayed-type hypersensitivity by VH and I-A-region genes
复制标题
对 4-羟基-3-硝基苯乙酰基的半抗原特异性 T 细胞反应。
DOI：
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发表时间：
1979
影响因子：
15.3
通讯作者：
M. Dorf
中科院分区：
文献类型：
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作者：
J. Weinberger;M. Greene;B. Benacerraf;M. Dorf
半抗原特异性延迟型超敏反应（DTH）在多个品系小鼠中被诱导。未携带Ig1b重链基团的（4-羟基-3-硝基苯）乙酰-牛γ球蛋白（NP- bgg）引物小鼠对NP牛血清白蛋白（BSA）有NP特异性的DTH反应，而对（4-羟基-5-碘-3-硝基苯）乙酰-牛血清白蛋白（NIP-BSA）没有反应。携带Ig1b同种异型的np - bgg引物小鼠，包括SJL，对NP-BSA或NIP-BSA均有应答。一株交叉反应菌株C57BL/6与另外两株非交叉反应菌株之间的F1杂交呈交叉反应。nip -交叉反应性DTH反应的遗传定位将该性状定位在Ig1b重链连锁组的vh区域。t细胞抗np反应的精细特异性模式及其遗传定位与报道的体液异位抗np反应的精细特异性和定位数据相似。关于在不同菌株组合之间转移np特异性DTH能力的过继转移研究表明，T细胞供体和受体必须至少在I-A亚区具有同源性。当从对NIP有交叉反应性的菌株转移到NP特异性反应性时，受体也对NP和NIP都有反应。讨论了两个不同的遗传区域VH和H-2对NP引发的T细胞dth反应群体的控制意义。
Hapten-specific delayed-type hypersensitivity (DTH) was induced in several strains of mice. (4-hydroxy-3-nitrophenyl)acetyl-bovine gamma globulin (NP-BGG)-primed mice which did not bear the Ig1b heavy-chain linkage group made a NP-specific DTH response when challenged with NP bovine serum albumin (BSA) and failed to respond to challenge with (4- hydroxy-5-iodo-3-nitrophenyl)acetyl-bovine serum albumin (NIP-BSA). Strains of NP-BGG-primed mice bearing the Ig1b allotype, including SJL, responded to challenges of either NP-BSA or NIP-BSA. F1 hybrids between a cross-reactive strain, C57BL/6, and two other noncross-reactive strains were cross-reactive. Genetic mapping of the NIP-cross-reactive DTH response localized the trait to the VH-region of the Ig1b heavy- chain linkage group. The fine-specificity pattern of the T-cell anti-NP response, and the genetic mapping of this trait, were analogous to the reported fine specificity and mapping data of the humoral heteroclitic anti-NP response. Adoptive transfer studies on the ability to transfer NP-specific DTH between various strain combinations showed that the T- cell donors and the recipient must have homology for at least the I-A subregion. Whenever NP-specific reactivity was transferred from a strain which cross-reactively responded to NIP, the recipient also responded to both NP and NIP. The implications of the control of NP- primed DTH-reactive populations of T cells by two distinct genetic regions, VH and H-2, were discussed.