Metabolite profiling of the short-term responses of rice leaves (Oryza sativa. cv. Ilmi) cultivated under different LED lights and its correlations with antioxidant activities
Metabolite profiling of the short-term responses of rice leaves (Oryza sativa. cv. Ilmi) cultivated under different LED lights and its correlations with antioxidant activities
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DOI：
10.1016/j.plantsci.2013.05.004
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发表时间：
2013-09-01
期刊：
影响因子：
5.2
通讯作者：
Lee, Choong Hwan
中科院分区：
文献类型：
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作者：
Jung, Eun Sung;Lee, Sarah;Lee, Choong Hwan
水稻叶片（Oryza sativa cv.通过气相色谱-离子阱-质谱（GC-IT-MS）和超高效液相色谱-四极杆-飞行时间-质谱（UPLC-Q-TOF-MS）结合多变量分析，研究了不同发光二极管（LED）光（蓝光（B）、绿色（G）、红光（R）、白色（W）、色光（S））对小鼠的短期响应。偏最小二乘判别分析（PLS-DA）显示了除G和W外的每个光生长样品的清晰分组模式。选择32种初级代谢产物和11种次级代谢产物，并使用热图进行可视化。水稻叶片抗氧化活性的大小顺序为B = W = G > R > S，其中异Orientin-2“”-O-葡萄糖苷、异Orientin-2“”-O-葡萄糖苷、异Orientin-2“”-O-（6“”-rho-香豆酰基）-葡萄糖苷和异甘草素-2“-O-葡萄糖苷的相对差异相似，相关系数较高。根据B和R的正交投影潜在结构判别分析（OPLS-DA），B中氨基酸、有机酸、脂肪酸和黄酮苷含量较高，而R中葡萄糖和果糖含量较高。(c)爱思唯尔爱尔兰有限公司版权所有。
Metabolite profiling of rice leaves (Oryza sativa cv. Ilmi) was performed to investigate the short-term responses to different light-emitting diode (LED) lights, blue (B), green (G), red (R), white (W), shade (S), by using gas chromatography-ion trap-mass spectrometry (GC-IT-MS) and ultra-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) with multivariate analysis. Clear grouping patterns of each light-grown sample, except G and W, were shown in partial least squares-discriminant analysis (PLS-DA). Thirty-two primary metabolites and eleven secondary metabolites were selected and visualized using heatmap. Antioxidant activities of rice leaves followed the order B = W = G > R > S and isoorientin-2 ''-O-glucoside, isoorientin-2 ''-O-glucoside, isoorientin-2 ''-O-(6'''-rho-coumaroyl)-glucoside, and isoscoparin-2 ''-O-glucoside showed similar relative differences and had higher Pearson's correlation coefficients than other metabolites in correlation network. According to the orthogonal projection to latent structures-discriminant analysis (OPLS-DA) between B and R, the levels of amino acids, organic acids, fatty acids, and flavonoid glycosides were relatively high in B, whereas the glucose and fructose levels were high in R. (c) Elsevier Ireland Ltd. All rights reserved.